TY - JOUR
T1 - Pulmonary intravascular volume can be used for dose calculation in isolated lung perfusion
AU - van Putte, Bart P.
AU - Huisman, Albert
AU - Hendriks, Jeroen M. H.
AU - van Schil, Paul E. Y.
AU - van Boven, Wim J.
AU - Schramel, Franz
AU - Nijkamp, Frans
AU - Folkerts, Gert
PY - 2005
Y1 - 2005
N2 - Introduction: Isolated lung perfusion (ILuP) is an experimental surgical technique for the treatment of pulmonary metastases. Phase I trials showed a wide range in drug lung levels. This may be due to the variance of lung size and pulmonary intravascular volume (PIV). Therefore, we developed a method to assess PIV and investigated the relation of PIV and dry lung weight (DLW). Material and methods: Thirty-two rats of 555 +/- 8 and 199 +/- 5 g underwent left ILuP two, four and eight minutes. Venous effluent was analyzed for haemoglobin, red blood cells (RBC), leucocytes, platelets, albumin and creatinine. PIV was calculated by dividing the product of perfusate volume and post-ILuP parameter by the difference between post-ILuP and pre-ILuP parameter. Results: No significant differences in PIV for all perfusion times were noted between the different variables (P=0.14). Based on haemoglobin (P <0.0009), RBC (P=0.006), leucocytes (P=0.0003), platelets (P=0.017) and creatinine (P=0.003) analysis, PIV was significantly smaller in rats of 199 g while PIV/DLW ratio was not significantly different. Conclusion: Because PIV/DLW ratio is independent of body weight, we advocate PIV calculation using haemoglobin and RBC as an excellent parameter for drug dose calculation during ILuP intraoperatively in order to achieve more reproducible local drug levels and higher efficacy. (c) 2005 Elsevier B.V. All rights reserved
AB - Introduction: Isolated lung perfusion (ILuP) is an experimental surgical technique for the treatment of pulmonary metastases. Phase I trials showed a wide range in drug lung levels. This may be due to the variance of lung size and pulmonary intravascular volume (PIV). Therefore, we developed a method to assess PIV and investigated the relation of PIV and dry lung weight (DLW). Material and methods: Thirty-two rats of 555 +/- 8 and 199 +/- 5 g underwent left ILuP two, four and eight minutes. Venous effluent was analyzed for haemoglobin, red blood cells (RBC), leucocytes, platelets, albumin and creatinine. PIV was calculated by dividing the product of perfusate volume and post-ILuP parameter by the difference between post-ILuP and pre-ILuP parameter. Results: No significant differences in PIV for all perfusion times were noted between the different variables (P=0.14). Based on haemoglobin (P <0.0009), RBC (P=0.006), leucocytes (P=0.0003), platelets (P=0.017) and creatinine (P=0.003) analysis, PIV was significantly smaller in rats of 199 g while PIV/DLW ratio was not significantly different. Conclusion: Because PIV/DLW ratio is independent of body weight, we advocate PIV calculation using haemoglobin and RBC as an excellent parameter for drug dose calculation during ILuP intraoperatively in order to achieve more reproducible local drug levels and higher efficacy. (c) 2005 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.ejcts.2005.06.044
DO - https://doi.org/10.1016/j.ejcts.2005.06.044
M3 - Article
C2 - 16135409
SN - 1010-7940
VL - 28
SP - 594
EP - 598
JO - European journal of cardio-thoracic surgery
JF - European journal of cardio-thoracic surgery
IS - 4
ER -