TY - JOUR
T1 - Quantitative 3D analysis of tissue damage in a rat model of microembolization
AU - Georgakopoulou, Theodosia
AU - van der Wijk, Anne-Eva
AU - Bakker, Erik N. T. P.
AU - vanBavel, Ed
AU - Majoie, Charles
AU - Marquering, Henk
AU - van Bavel, Ed
AU - Hoekstra, Alfons
AU - Dippel, Diederik
AU - Lingsma, Hester
AU - van der Lugt, Aad
AU - Samuels, Noor
AU - Boodt, Nikki
AU - Roos, Yvo
AU - de Meyer, Simon
AU - Staessens, Senna
AU - Vandelanotte, Sarah
AU - Konduri, Praneeta
AU - Terreros, Nerea Arrarte
AU - Chopard, Bastien
AU - Raynaud, Franck
AU - Petkantchin, Remy
AU - Panteleev, Mikhail
AU - Shibeko, Alexey
AU - Boudjeltia, Karim Zouaoui
AU - Blanc-Guillemaud, Vanessa
AU - Migliavacca, Francesco
AU - Dubini, Gabriele
AU - Luraghi, Giulia
AU - Matas, Jose Felix Rodriguez
AU - Bridio, Sara
AU - Mc Garry, Patrick
AU - Gilvarry, Michael
AU - McCarthy, Ray
AU - Moerman, Kevin
AU - Fereidoonnezhad, Behrooz
AU - Dwivedi, Anushree
AU - Duffy, Sharon
AU - Payne, Stephen
AU - Jozsa, Tamas
AU - Georgakopoulou, Sissy
AU - Padmos, Raymond
AU - Azizi, Victor
AU - Miller, Claire
AU - van der Kolk, Max
AU - INSIST investigators
N1 - Funding Information: This project was funded from the European Union's Horizon 2020 research and innovation program under grant agreement No 777072 (INSIST). AEvdW was funded by Neuroscience Amsterdam (project number NDIS-2019-03) Funding Information: Sources of funding. This project was funded from the European Union's Horizon 2020 research and innovation program under grant agreement No 777072 (INSIST). AEvdW was funded by Neuroscience Amsterdam (project number NDIS-2019-03), Author contributions. Declaration of Competing Interest. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. ENTPB, EvB, AEvdW and TG designed the experiments; TG and AEvdW performed the surgeries; TG and EvB analyzed the data and wrote the manuscript; AEvdW, ENTPB and EvB contributed to editing of the manuscript. All authors read and approved the final version of the manuscript. Publisher Copyright: © 2021 The Authors
PY - 2021/11/9
Y1 - 2021/11/9
N2 - There is a discrepancy between successful recanalization and good clinical outcome after endovascular treatment (EVT) in acute ischemic stroke patients. During removal of a thrombus, a shower of microemboli may release and lodge to the distal circulation. The objective of this study was to determine the extent of damage on brain tissue caused by microemboli. In a rat model of microembolization, a mixture of microsphere (MS) sizes (15, 25 and 50 µm diameter) was injected via the left internal carotid artery. A 3D image of the left hemisphere was reconstructed and a point-pattern spatial analysis was applied based on G- and K-functions to unravel the spatial correlation between MS and the induced hypoxia or infarction. We show a spatial correlation between MS and hypoxia or infarction spreading up to a distance of 1000–1500 µm. These results imply that microemboli, which individually may not always be harmful, can interact and result in local areas of hypoxia or even infarction when lodged in large numbers.
AB - There is a discrepancy between successful recanalization and good clinical outcome after endovascular treatment (EVT) in acute ischemic stroke patients. During removal of a thrombus, a shower of microemboli may release and lodge to the distal circulation. The objective of this study was to determine the extent of damage on brain tissue caused by microemboli. In a rat model of microembolization, a mixture of microsphere (MS) sizes (15, 25 and 50 µm diameter) was injected via the left internal carotid artery. A 3D image of the left hemisphere was reconstructed and a point-pattern spatial analysis was applied based on G- and K-functions to unravel the spatial correlation between MS and the induced hypoxia or infarction. We show a spatial correlation between MS and hypoxia or infarction spreading up to a distance of 1000–1500 µm. These results imply that microemboli, which individually may not always be harmful, can interact and result in local areas of hypoxia or even infarction when lodged in large numbers.
KW - Endovascular therapy
KW - Hypoxia
KW - Incomplete microvascular reperfusion
KW - Microembolization
KW - Spatial analysis
KW - Stroke
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85114684544&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/34509910
UR - http://www.scopus.com/inward/record.url?scp=85114684544&partnerID=8YFLogxK
UR - https://pure.uva.nl/ws/files/67543225/1_s2.0_S0021929021004917_mmc1.docx
UR - https://pure.uva.nl/ws/files/66851381/INSIST_investigators.pdf
U2 - https://doi.org/10.1016/j.jbiomech.2021.110723
DO - https://doi.org/10.1016/j.jbiomech.2021.110723
M3 - Article
C2 - 34509910
SN - 0021-9290
VL - 128
JO - Journal of Biomechanics
JF - Journal of Biomechanics
M1 - 110723
ER -