TY - JOUR
T1 - Quantitative and dynamic assessment of the contribution of the ER to phagosome formation
AU - Touret, Nicolas
AU - Paroutis, Paul
AU - Terebiznik, Mauricio
AU - Harrison, Rene E.
AU - Trombetta, Sergio
AU - Pypaert, Marc
AU - Chow, Amy
AU - Jiang, Aimin
AU - Shaw, James
AU - Yip, Christopher
AU - Moore, Hsiao-Ping
AU - van der Wel, Nicole
AU - Houben, Diane
AU - Peters, Peter J.
AU - de Chastellier, Chantal
AU - Mellman, Ira
AU - Grinstein, Sergio
PY - 2005
Y1 - 2005
N2 - Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate hypotheses, we used a combination of biochemical, fluorescence imaging, and electron microscopy techniques to quantitatively and dynamically assess the contribution of the plasmalemma and of the ER to phagosome formation and maturation. We could not verify even a transient physical continuity between the ER and the plasma membrane, nor were we able to detect a significant contribution of the ER to forming or maturing phagosomes in either macrophages or dendritic cells. Instead, our data indicate that the plasma membrane is the main constituent of nascent and newly formed phagosomes, which are progressively remodeled by fusion with endosomal and eventually lysosomal compartments as phagosomes mature into acidic, degradative organelles
AB - Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate hypotheses, we used a combination of biochemical, fluorescence imaging, and electron microscopy techniques to quantitatively and dynamically assess the contribution of the plasmalemma and of the ER to phagosome formation and maturation. We could not verify even a transient physical continuity between the ER and the plasma membrane, nor were we able to detect a significant contribution of the ER to forming or maturing phagosomes in either macrophages or dendritic cells. Instead, our data indicate that the plasma membrane is the main constituent of nascent and newly formed phagosomes, which are progressively remodeled by fusion with endosomal and eventually lysosomal compartments as phagosomes mature into acidic, degradative organelles
U2 - https://doi.org/10.1016/j.cell.2005.08.018
DO - https://doi.org/10.1016/j.cell.2005.08.018
M3 - Article
C2 - 16213220
SN - 0092-8674
VL - 123
SP - 157
EP - 170
JO - Cell
JF - Cell
IS - 1
ER -