Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Annemarie Nadort; Rutger G. Woolthuis; Ton G. van Leeuwen; Dirk J. Faber

doi:https://doi.org/10.1364/BOE.4.002347

Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Annemarie Nadort, Rutger G. Woolthuis, Ton G. van Leeuwen, Dirk J. Faber

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources. (C) 2013 Optical Society of America

Original language	English
Pages (from-to)	2347-2361
Journal	Biomedical Optics Express
Volume	4
Issue number	11
DOIs	https://doi.org/10.1364/BOE.4.002347
Publication status	Published - 2013

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https://doi.org/10.1364/BOE.4.002347

Cite this

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abstract = "We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources. (C) 2013 Optical Society of America",

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AB - We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources. (C) 2013 Optical Society of America

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