TY - JOUR
T1 - Quantum Dot and Cy5.5 Labeled Nanoparticles to Investigate Lipoprotein Biointeractions via Förster Resonance Energy Transfer
AU - Skajaa, Torjus
AU - Zhao, Yiming
AU - van den Heuvel, Dave J.
AU - Gerritsen, Hans C.
AU - Cormode, David P.
AU - Koole, Rolf
AU - van Schooneveld, Matti M.
AU - Post, Jan Andries
AU - Fisher, Edward A.
AU - Fayad, Zahi A.
AU - de Mello Donega, Celso
AU - Meijerink, Andries
AU - Mulder, Willem J. M.
PY - 2010
Y1 - 2010
N2 - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein-lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells
AB - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein-lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells
U2 - https://doi.org/10.1021/nl1037903
DO - https://doi.org/10.1021/nl1037903
M3 - Article
C2 - 21087054
SN - 1530-6984
VL - 10
SP - 5131
EP - 5138
JO - Nano letters
JF - Nano letters
IS - 12
ER -