TY - JOUR
T1 - Rapid SARS-CoV-2 whole-genome sequencing and analysis for informed public health decision-making in the Netherlands
AU - The Dutch-Covid-19 response team
AU - Oude Munnink, Bas B.
AU - Nieuwenhuijse, David F.
AU - Stein, Mart
AU - O’Toole, Áine
AU - Haverkate, Manon
AU - Mollers, Madelief
AU - Kamga, Sandra K.
AU - Schapendonk, Claudia
AU - Pronk, Mark
AU - Lexmond, Pascal
AU - van der Linden, Anne
AU - Bestebroer, Theo
AU - Chestakova, Irina
AU - Overmars, Ronald J.
AU - van Nieuwkoop, Stefan
AU - Molenkamp, Richard
AU - van der Eijk, Annemiek A.
AU - GeurtsvanKessel, Corine
AU - Vennema, Harry
AU - Meijer, Adam
AU - Rambaut, Andrew
AU - van Dissel, Jaap
AU - Sikkema, Reina S.
AU - Timen, Aura
AU - Koopmans, Marion
AU - Oudehuis, G. J. A. P. M.
AU - Schinkel, Janke
AU - Kluytmans, Jan
AU - Kluytmans-van den Bergh, Marjolein
AU - van den Bijllaardt, Wouter
AU - Berntvelsen, Robbert G.
AU - van Rijen, Miranda M. L.
AU - Schneeberger, Peter
AU - Pas, Suzan
AU - Diederen, Bram M.
AU - Bergmans, Anneke M. C.
AU - van der Eijk, P. A. Verspui
AU - Verweij, Jaco
AU - Buiting, Anton G. N.
AU - Streefkerk, Roel
AU - Aldenkamp, A. P.
AU - de Man, P.
AU - Koelemal, J. G. M.
AU - Ong, D.
AU - Paltansing, S.
AU - Veassen, N.
AU - Sleven, Jacqueline
AU - Bakker, Leendert
AU - Brockhoff, Heinrich
AU - van Tienen, Carla
PY - 2020/9/1
Y1 - 2020/9/1
N2 - In late December 2019, a cluster of cases of pneumonia of unknown etiology were reported linked to a market in Wuhan, China1. The causative agent was identified as the species Severe acute respiratory syndrome-related coronavirus and was named SARS-CoV-2 (ref. 2). By 16 April the virus had spread to 185 different countries, infected over 2,000,000 people and resulted in over 130,000 deaths3. In the Netherlands, the first case of SARS-CoV-2 was notified on 27 February. The outbreak started with several different introductory events from Italy, Austria, Germany and France followed by local amplification in, and later also outside, the south of the Netherlands. The combination of near to real-time whole-genome sequence analysis and epidemiology resulted in reliable assessments of the extent of SARS-CoV-2 transmission in the community, facilitating early decision-making to control local transmission of SARS-CoV-2 in the Netherlands. We demonstrate how these data were generated and analyzed, and how SARS-CoV-2 whole-genome sequencing, in combination with epidemiological data, was used to inform public health decision-making in the Netherlands.
AB - In late December 2019, a cluster of cases of pneumonia of unknown etiology were reported linked to a market in Wuhan, China1. The causative agent was identified as the species Severe acute respiratory syndrome-related coronavirus and was named SARS-CoV-2 (ref. 2). By 16 April the virus had spread to 185 different countries, infected over 2,000,000 people and resulted in over 130,000 deaths3. In the Netherlands, the first case of SARS-CoV-2 was notified on 27 February. The outbreak started with several different introductory events from Italy, Austria, Germany and France followed by local amplification in, and later also outside, the south of the Netherlands. The combination of near to real-time whole-genome sequence analysis and epidemiology resulted in reliable assessments of the extent of SARS-CoV-2 transmission in the community, facilitating early decision-making to control local transmission of SARS-CoV-2 in the Netherlands. We demonstrate how these data were generated and analyzed, and how SARS-CoV-2 whole-genome sequencing, in combination with epidemiological data, was used to inform public health decision-making in the Netherlands.
UR - http://www.scopus.com/inward/record.url?scp=85087946148&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41591-020-0997-y
DO - https://doi.org/10.1038/s41591-020-0997-y
M3 - Article
C2 - 32678356
SN - 1078-8956
VL - 26
SP - 1405
EP - 1410
JO - Nature medicine
JF - Nature medicine
IS - 9
ER -