TY - JOUR
T1 - Reconstitution of EBV latent but not lytic antigen-specific CD4(+) and CD8(+) T cells after HIV treatment with highly active antiretroviral therapy
AU - Piriou, Erwan
AU - Jansen, Christine A.
AU - van Dort, Karel
AU - de Cuyper, Iris
AU - Nanlohy, Nening M.
AU - Lange, Joep M. A.
AU - van Oers, Marinus H. J.
AU - Miedema, Frank
AU - van Baarle, Debbie
PY - 2005
Y1 - 2005
N2 - The incidence of (EBV-rlelated) malignancies in HIV-infected subjects has declined since the introduction of highly active antiretroviral therapy (HAART). To investigate the effect of HAART on EBV infection, we performed a longitudinal analysis of the T cell response to both a latent and a lytic Ag and EBV viral load in 10 subjects from early in HIV infection up to 5 years after HAART. All individuals responded to HAART by a decline in HIV viral load, a restoration of total CD4(+) T cell numbers, and a decline in T cell immune activation. Despite this, EBV load remained unaltered, even after 5 years of therapy, although a decline in both CD4(+) and CD8(+) T cells specific for the lytic EBV protein BZLF1 suggested a decreased EBV reactivation rate. In contrast, latent EBV Ag EBNA1-specific CD4(+) and CD8(+) T cell responses were restored after 5 years of treatment to levels comparable to healthy individuals. In two individuals who were treated by HAART late during HIV progression, a lymphoma developed shortly after initiation of HAART, despite restoration of EBV-specific CD4(+) and CD8(+) T cells. In conclusion, long-term HAART does not alter the EBV DNA load, but does lead to a restoration of EBNA1-specific T cell responses, which might allow better control of EBV-infected cells when applied early enough during HIV infection
AB - The incidence of (EBV-rlelated) malignancies in HIV-infected subjects has declined since the introduction of highly active antiretroviral therapy (HAART). To investigate the effect of HAART on EBV infection, we performed a longitudinal analysis of the T cell response to both a latent and a lytic Ag and EBV viral load in 10 subjects from early in HIV infection up to 5 years after HAART. All individuals responded to HAART by a decline in HIV viral load, a restoration of total CD4(+) T cell numbers, and a decline in T cell immune activation. Despite this, EBV load remained unaltered, even after 5 years of therapy, although a decline in both CD4(+) and CD8(+) T cells specific for the lytic EBV protein BZLF1 suggested a decreased EBV reactivation rate. In contrast, latent EBV Ag EBNA1-specific CD4(+) and CD8(+) T cell responses were restored after 5 years of treatment to levels comparable to healthy individuals. In two individuals who were treated by HAART late during HIV progression, a lymphoma developed shortly after initiation of HAART, despite restoration of EBV-specific CD4(+) and CD8(+) T cells. In conclusion, long-term HAART does not alter the EBV DNA load, but does lead to a restoration of EBNA1-specific T cell responses, which might allow better control of EBV-infected cells when applied early enough during HIV infection
U2 - https://doi.org/10.4049/jimmunol.175.3.2010
DO - https://doi.org/10.4049/jimmunol.175.3.2010
M3 - Article
C2 - 16034146
SN - 0022-1767
VL - 175
SP - 2010
EP - 2017
JO - Journal of immunology (Baltimore, Md.
JF - Journal of immunology (Baltimore, Md.
IS - 3
ER -