TY - JOUR
T1 - Recovery of Hypoxic Regions in a Rat Model of Microembolism
AU - Georgakopoulou, Theodosia
AU - van der Wijk, Anne-Eva
AU - Bakker, Erik N. T. P.
AU - vanBavel, Ed
N1 - Funding Information: This project was funded from the European Union's Horizon 2020 research and innovation program under grant agreement No. 777072 (INSIST). Publisher Copyright: © 2021 The Authors
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Objectives: Endovascular treatment (EVT) has become the standard of care for acute ischemic stroke. Despite successful recanalization, a limited subset of patients benefits from the new treatment. Human MRI studies have shown that during removal of the thrombus, a shower of microclots is released from the initial thrombus, possibly causing new ischemic lesions. The aim of the current study is to quantify tissue damage following microembolism. Materials and methods: In a rat model, microembolism was generated by injection of a mixture of polystyrene fluorescent microspheres (15, 25 and 50 µm in diameter). The animals were killed at three time-points: day 1, 3 or 7. AMIRA and IMARIS software was used for 3D reconstruction of brain structure and damage, respectively. Conclusions: Microembolism induces ischemia, hypoxia and infarction. Infarcted areas persist, but hypoxic regions recover over time suggesting that repair processes in the brain rescue the regions at risk.
AB - Objectives: Endovascular treatment (EVT) has become the standard of care for acute ischemic stroke. Despite successful recanalization, a limited subset of patients benefits from the new treatment. Human MRI studies have shown that during removal of the thrombus, a shower of microclots is released from the initial thrombus, possibly causing new ischemic lesions. The aim of the current study is to quantify tissue damage following microembolism. Materials and methods: In a rat model, microembolism was generated by injection of a mixture of polystyrene fluorescent microspheres (15, 25 and 50 µm in diameter). The animals were killed at three time-points: day 1, 3 or 7. AMIRA and IMARIS software was used for 3D reconstruction of brain structure and damage, respectively. Conclusions: Microembolism induces ischemia, hypoxia and infarction. Infarcted areas persist, but hypoxic regions recover over time suggesting that repair processes in the brain rescue the regions at risk.
KW - Hypoxia
KW - Infarction
KW - Ischemia
KW - Microembolism
KW - Stroke
UR - http://www.scopus.com/inward/record.url?scp=85102871843&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.105739
DO - https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.105739
M3 - Article
C2 - 33765634
SN - 1052-3057
VL - 30
JO - Journal of Stroke and Cerebrovascular Diseases
JF - Journal of Stroke and Cerebrovascular Diseases
IS - 6
M1 - 105739
ER -