Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS)

Rahel M. Büttler; Jiska S. Peper; Eveline A. Crone; Eef G. W. Lentjes; Marinus A. Blankenstein; Annemieke C. Heijboer

doi:https://doi.org/10.1016/j.cca.2016.02.015

Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS)

Rahel M. Büttler, Jiska S. Peper, Eveline A. Crone, Eef G. W. Lentjes, Marinus A. Blankenstein, Annemieke C. Heijboer

Research output: Contribution to journal › Article › Academic › peer-review

24 Citations (Scopus)

Abstract

The measurement of testosterone in saliva is an attractive alternative to serum analysis due to the simple and non-invasive sample collection. In children and adolescents salivary testosterone is mainly measured to investigate whether puberty has started or not. This study aimed to establish reference values for salivary testosterone during puberty in boys and girls. We measured salivary testosterone using ID-LC-MS/MS in a cohort of 131 girls and 123 boys of whom each had salivary testosterone measured at two time points during puberty. Salivary testosterone concentrations start to increase with the start of puberty around eight years and continuously increase up to adult concentrations in the following ten years. Reference values were calculated using the Lambda-Mu-Sigma (LMS)-curve fitting method and provided per year from 8 to 26 years of age in boys and girls. These reference ranges may help clinicians and researchers to interpret salivary testosterone results in both individual patients and study subjects

Original language	English
Pages (from-to)	15-18
Journal	Clinica chimica acta; international journal of clinical chemistry
Volume	456
DOIs	https://doi.org/10.1016/j.cca.2016.02.015
Publication status	Published - 1 May 2016

Keywords

Adolescents
Reference values
Salivary testosterone

Access to Document

https://doi.org/10.1016/j.cca.2016.02.015

Cite this

Büttler, R. M., Peper, J. S., Crone, E. A., Lentjes, E. G. W., Blankenstein, M. A., & Heijboer, A. C. (2016). Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS). Clinica chimica acta; international journal of clinical chemistry, 456, 15-18. https://doi.org/10.1016/j.cca.2016.02.015

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abstract = "The measurement of testosterone in saliva is an attractive alternative to serum analysis due to the simple and non-invasive sample collection. In children and adolescents salivary testosterone is mainly measured to investigate whether puberty has started or not. This study aimed to establish reference values for salivary testosterone during puberty in boys and girls. We measured salivary testosterone using ID-LC-MS/MS in a cohort of 131 girls and 123 boys of whom each had salivary testosterone measured at two time points during puberty. Salivary testosterone concentrations start to increase with the start of puberty around eight years and continuously increase up to adult concentrations in the following ten years. Reference values were calculated using the Lambda-Mu-Sigma (LMS)-curve fitting method and provided per year from 8 to 26 years of age in boys and girls. These reference ranges may help clinicians and researchers to interpret salivary testosterone results in both individual patients and study subjects",

keywords = "Adolescents, Reference values, Salivary testosterone",

author = "B{\"u}ttler, {Rahel M.} and Peper, {Jiska S.} and Crone, {Eveline A.} and Lentjes, {Eef G. W.} and Blankenstein, {Marinus A.} and Heijboer, {Annemieke C.}",

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Büttler, RM, Peper, JS, Crone, EA, Lentjes, EGW, Blankenstein, MA & Heijboer, AC 2016, 'Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS)', Clinica chimica acta; international journal of clinical chemistry, vol. 456, pp. 15-18. https://doi.org/10.1016/j.cca.2016.02.015

Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS). / Büttler, Rahel M.; Peper, Jiska S.; Crone, Eveline A. et al.
In: Clinica chimica acta; international journal of clinical chemistry, Vol. 456, 01.05.2016, p. 15-18.

Research output: Contribution to journal › Article › Academic › peer-review

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Büttler RM, Peper JS, Crone EA, Lentjes EGW, Blankenstein MA , Heijboer AC. Reference values for salivary testosterone in adolescent boys and girls determined using Isotope-Dilution Liquid-Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS). Clinica chimica acta; international journal of clinical chemistry. 2016 May 1;456:15-18. doi: https://doi.org/10.1016/j.cca.2016.02.015