TY - JOUR
T1 - Release of filamentous and spherical influenza a virus is not restricted by tetherin
AU - Bruce, Emily A.
AU - Abbink, Truus E.
AU - Wise, Helen M.
AU - Rollason, Ruth
AU - Galao, Rui Pedro
AU - Banting, George
AU - Neil, Stuart J.
AU - Digard, Paul
PY - 2012
Y1 - 2012
N2 - The cellular protein tetherin is thought to act as a 'leash' that anchors many enveloped viruses to the plasma membrane and prevents their release. We found that replication of multiple strains of influenza A virus was generally insensitive to alteration of tetherin levels, as assessed by output titre or scanning electron microscopy of cell-associated virions. This included human, swine, avian and equine isolates, strains that form filamentous or spherical particles and viruses that lack the M2 or NS1 proteins. Levels of cell-surface tetherin were not reduced by influenza infection, but tetherin and the viral haemagglutinin co-localized on the plasma membrane. However, tetherin could not be detected in filamentous virions, suggesting that influenza may possess a mechanism to exclude it from virions. Overall, if influenza does encode a specific antagonist of tetherin, it is not M2 or NS1 and we find no evidence for a role in host range specificity.
AB - The cellular protein tetherin is thought to act as a 'leash' that anchors many enveloped viruses to the plasma membrane and prevents their release. We found that replication of multiple strains of influenza A virus was generally insensitive to alteration of tetherin levels, as assessed by output titre or scanning electron microscopy of cell-associated virions. This included human, swine, avian and equine isolates, strains that form filamentous or spherical particles and viruses that lack the M2 or NS1 proteins. Levels of cell-surface tetherin were not reduced by influenza infection, but tetherin and the viral haemagglutinin co-localized on the plasma membrane. However, tetherin could not be detected in filamentous virions, suggesting that influenza may possess a mechanism to exclude it from virions. Overall, if influenza does encode a specific antagonist of tetherin, it is not M2 or NS1 and we find no evidence for a role in host range specificity.
UR - http://www.scopus.com/inward/record.url?scp=84859837135&partnerID=8YFLogxK
U2 - https://doi.org/10.1099/vir.0.038778-0
DO - https://doi.org/10.1099/vir.0.038778-0
M3 - Article
C2 - 22258861
SN - 0022-1317
VL - 93
SP - 963
EP - 969
JO - Journal of general virology
JF - Journal of general virology
IS - 5
ER -