TY - JOUR
T1 - Removal of contaminating DNA from commercial nucleic acid extraction kit reagents
AU - Mohammadi, T.
AU - Reesink, H.W.
AU - Vandenbroucke-Grauls, C.M.J.E.
AU - Savelkoul, P.H.M.
PY - 2005
Y1 - 2005
N2 - Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained
AB - Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained
U2 - https://doi.org/10.1016/j.mimet.2004.11.018
DO - https://doi.org/10.1016/j.mimet.2004.11.018
M3 - Article
C2 - 15722157
SN - 0167-7012
VL - 61
SP - 285
EP - 288
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -