TY - JOUR
T1 - Replicon typing of plasmids in Escherichia coli producing extended-spectrum β-lactamases
AU - Marcadé, Geraldine
AU - Deschamps, Catherine
AU - Boyd, Anders
AU - Gautier, Valérie
AU - Picard, Bertrand
AU - Branger, Catherine
AU - Denamur, Erick
AU - Arlet, Guillaume
PY - 2009
Y1 - 2009
N2 - Objectives: Escherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in the emergence and spread of ESBLs in relation to ESBL type. Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli strains was analysed. The replicons carrying the ESBLs and the total plasmid content of the strains have been characterized by PCR replicon typing in relation to the type of ESBL. The ESBL replicons were then compared with the replicon content of E. coli strains carrying TEM-1 or inhibitor-resistant TEM (IRT) β-lactamases. Results: IncF plasmids were the most frequently carried replicons in our collection, but none carried TEM ESBL. Of TEM ESBLs, 67% were carried on IncA/C replicons except for TEM-52 genes, which were carried preferentially on IncI1 replicons. Although CTX-M enzymes can be carried by various replicons, the great majority of genes encoding CTX-M-14 and CTX-M-15 ESBLs were carried by IncF replicons, as were TEM-1 and IRT β-lactamases. Conclusions: Resistance genes borne by the narrow host-range IncF replicon spread readily as this replicon is well adapted to E. coli. This is observed for blaTEM-1 and blaCTX-M-15 and, to a lesser extent, for blaCTX-M-14. Transposition immunity seems to play an important role in the diffusion process. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
AB - Objectives: Escherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in the emergence and spread of ESBLs in relation to ESBL type. Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli strains was analysed. The replicons carrying the ESBLs and the total plasmid content of the strains have been characterized by PCR replicon typing in relation to the type of ESBL. The ESBL replicons were then compared with the replicon content of E. coli strains carrying TEM-1 or inhibitor-resistant TEM (IRT) β-lactamases. Results: IncF plasmids were the most frequently carried replicons in our collection, but none carried TEM ESBL. Of TEM ESBLs, 67% were carried on IncA/C replicons except for TEM-52 genes, which were carried preferentially on IncI1 replicons. Although CTX-M enzymes can be carried by various replicons, the great majority of genes encoding CTX-M-14 and CTX-M-15 ESBLs were carried by IncF replicons, as were TEM-1 and IRT β-lactamases. Conclusions: Resistance genes borne by the narrow host-range IncF replicon spread readily as this replicon is well adapted to E. coli. This is observed for blaTEM-1 and blaCTX-M-15 and, to a lesser extent, for blaCTX-M-14. Transposition immunity seems to play an important role in the diffusion process. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
KW - DNA Transposable Elements
KW - DNA, Bacterial/genetics
KW - Escherichia coli/enzymology
KW - Humans
KW - Plasmids/classification
KW - Polymerase Chain Reaction/methods
KW - Replicon
KW - beta-Lactamases/biosynthesis
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57649228983&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/18931389
U2 - https://doi.org/10.1093/jac/dkn428
DO - https://doi.org/10.1093/jac/dkn428
M3 - Article
C2 - 18931389
SN - 0305-7453
VL - 63
SP - 67
EP - 71
JO - The Journal of antimicrobial chemotherapy
JF - The Journal of antimicrobial chemotherapy
IS - 1
ER -