TY - JOUR
T1 - Reproducible diagnostic metabolites in plasma from typhoid fever patients in Asia and Africa
AU - Näsström, Elin
AU - Parry, Christopher M.
AU - Vu Thieu, Nga Tran
AU - Maude, Rapeephan R.
AU - de Jong, Hanna K.
AU - Fukushima, Masako
AU - Rzhepishevska, Olena
AU - Marks, Florian
AU - Panzner, Ursula
AU - Im, Justin
AU - Jeon, Hyonjin
AU - Park, Seeun
AU - Chaudhury, Zabeen
AU - Ghose, Aniruddha
AU - Samad, Rasheda
AU - van, Tan Trinh
AU - Johansson, Anders
AU - Dondorp, Arjen M.
AU - Thwaites, Guy E.
AU - Faiz, Abul
AU - Antti, Henrik
AU - Baker, Stephen
PY - 2017
Y1 - 2017
N2 - Salmonella Typhi is the causative agent of typhoid. Typhoid is diagnosed by blood culture, a method that lacks sensitivity, portability and speed. We have previously shown that specific metabolomic profiles can be detected in the blood of typhoid patients from Nepal (Näsström et al., 2014). Here, we performed mass spectrometry on plasma from Bangladeshi and Senegalese patients with culture confirmed typhoid fever, clinically suspected typhoid, and other febrile diseases including malaria. After applying supervised pattern recognition modelling, we could significantly distinguish metabolite profiles in plasma from the culture confirmed typhoid patients. After comparing the direction of change and degree of multivariate significance, we identified 24 metabolites that were consistently up- or down regulated in a further Bangladeshi/Senegalese validation cohort, and the Nepali cohort from our previous work. We have identified and validated a metabolite panel that can distinguish typhoid from other febrile diseases, providing a new approach for typhoid diagnostics
AB - Salmonella Typhi is the causative agent of typhoid. Typhoid is diagnosed by blood culture, a method that lacks sensitivity, portability and speed. We have previously shown that specific metabolomic profiles can be detected in the blood of typhoid patients from Nepal (Näsström et al., 2014). Here, we performed mass spectrometry on plasma from Bangladeshi and Senegalese patients with culture confirmed typhoid fever, clinically suspected typhoid, and other febrile diseases including malaria. After applying supervised pattern recognition modelling, we could significantly distinguish metabolite profiles in plasma from the culture confirmed typhoid patients. After comparing the direction of change and degree of multivariate significance, we identified 24 metabolites that were consistently up- or down regulated in a further Bangladeshi/Senegalese validation cohort, and the Nepali cohort from our previous work. We have identified and validated a metabolite panel that can distinguish typhoid from other febrile diseases, providing a new approach for typhoid diagnostics
U2 - https://doi.org/10.7554/eLife.15651
DO - https://doi.org/10.7554/eLife.15651
M3 - Article
C2 - 28483042
SN - 2050-084X
VL - 6
SP - e15651
JO - eLife
JF - eLife
ER -