TY - JOUR
T1 - Reprogramming of myeloid cells and their progenitors in patients with non-medullary thyroid carcinoma
AU - Rabold, Katrin
AU - Zoodsma, Martijn
AU - Grondman, Inge
AU - Kuijpers, Yunus
AU - Bremmers, Manita
AU - Jaeger, Martin
AU - Zhang, Bowen
AU - Hobo, Willemijn
AU - Bonenkamp, Han J.
AU - de Wilt, Johannes H. W.
AU - Janssen, Marcel J. R.
AU - Cornelissen, Lenneke A. M.
AU - van Engen-van Grunsven, Ilse C. H.
AU - Mulder, Willem J. M.
AU - Smit, Jan W. A.
AU - Adema, Gosse J.
AU - Netea, Mihai G.
AU - Li, Yang
AU - Xu, Cheng-Jian
AU - Netea-Maier, Romana T.
N1 - Funding Information: The authors are grateful to all volunteers for their participation in this study. The authors thank the Flow Cytometry Core Facility of Leiden University Medical Center for their support with flow cytometric measurements and the Human Genomics department of Leiden University Medical Center for their support with 10X single-cell assays. RTNM was supported by a grant (#10559) from the Dutch Cancer Society. K.R. was supported by a Radboud University Medical Center Junior researcher (Ph.D.) grant. Y.L. was supported by an ERC starting Grant (948207) and a Radboud University Medical Center Hypatia Grant (2018). C.J.X. was supported by Helmholtz Initiative and Networking Fund (1800167). Funding Information: The authors are grateful to all volunteers for their participation in this study. The authors thank the Flow Cytometry Core Facility of Leiden University Medical Center for their support with flow cytometric measurements and the Human Genomics department of Leiden University Medical Center for their support with 10X single-cell assays. RTNM was supported by a grant (#10559) from the Dutch Cancer Society. K.R. was supported by a Radboud University Medical Center Junior researcher (Ph.D.) grant. Y.L. was supported by an ERC starting Grant (948207) and a Radboud University Medical Center Hypatia Grant (2018). C.J.X. was supported by Helmholtz Initiative and Networking Fund (1800167). Publisher Copyright: © 2022, The Author(s).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Myeloid cells, crucial players in antitumoral defense, are affected by tumor-derived factors and treatment. The role of myeloid cells and their progenitors prior to tumor infiltration is poorly understood. Here we show single-cell transcriptomics and functional analyses of the myeloid cell lineage in patients with non-medullary thyroid carcinoma (TC) and multinodular goiter, before and after treatment with radioactive iodine compared to healthy controls. Integrative data analysis indicates that monocytes of TC patients have transcriptional upregulation of antigen presentation, reduced cytokine production capacity, and overproduction of reactive oxygen species. Interestingly, these cancer-related pathological changes are partially removed upon treatment. In bone marrow, TC patients tend to shift from myelopoiesis towards lymphopoiesis, reflected in transcriptional differences. Taken together, distinct transcriptional and functional changes in myeloid cells arise before their infiltration of the tumor and are already initiated in bone marrow, which suggests an active role in forming the tumor immune microenvironment.
AB - Myeloid cells, crucial players in antitumoral defense, are affected by tumor-derived factors and treatment. The role of myeloid cells and their progenitors prior to tumor infiltration is poorly understood. Here we show single-cell transcriptomics and functional analyses of the myeloid cell lineage in patients with non-medullary thyroid carcinoma (TC) and multinodular goiter, before and after treatment with radioactive iodine compared to healthy controls. Integrative data analysis indicates that monocytes of TC patients have transcriptional upregulation of antigen presentation, reduced cytokine production capacity, and overproduction of reactive oxygen species. Interestingly, these cancer-related pathological changes are partially removed upon treatment. In bone marrow, TC patients tend to shift from myelopoiesis towards lymphopoiesis, reflected in transcriptional differences. Taken together, distinct transcriptional and functional changes in myeloid cells arise before their infiltration of the tumor and are already initiated in bone marrow, which suggests an active role in forming the tumor immune microenvironment.
UR - http://www.scopus.com/inward/record.url?scp=85140071662&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41467-022-33907-4
DO - https://doi.org/10.1038/s41467-022-33907-4
M3 - Article
C2 - 36257966
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 6149
ER -