TY - JOUR
T1 - Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children
AU - Bella, Chiara Della
AU - Venturini, Elisabetta
AU - Devente, Savannah
AU - Piccini, Paola
AU - Tapinassi, Simona
AU - Bianchi, Leila
AU - Grassi, Alessia
AU - Benagiano, Marisa
AU - Alnwaisri, Heba F. Mustafa
AU - Montagnani, Carlotta
AU - Chiappini, Elena
AU - Bitter, Wilbert
AU - D’Elios, Mario M.
AU - de Martino, Maurizio
AU - Galli, Luisa
AU - D'Elios, Mario Milco
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.
AB - Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.
KW - Children
KW - ELISPOT
KW - Lymphadenitis
KW - Mycobacterium avium
KW - Nontuberculous mycobacteria
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062794609&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30834995
UR - http://www.scopus.com/inward/record.url?scp=85062794609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062794609&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s10096-019-03506-1
DO - https://doi.org/10.1007/s10096-019-03506-1
M3 - Article
C2 - 30834995
SN - 0934-9723
VL - 38
SP - 1113
EP - 1122
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
ER -
