TY - JOUR
T1 - Separate roles for antigen recognition and lymph node inflammation in CD8+ memory T cell formation
AU - Fransen, Marieke F.
AU - van Stipdonk, Marianne J.
AU - Sluijter, Marjolein
AU - Schoenberger, Stephen P.
AU - Melief, Cornelis J.
AU - Offringa, Rienk
PY - 2010
Y1 - 2010
N2 - Priming of naive CD8+ T cells by pathogens or vaccines generally involves their interaction with Ag-loaded dendritic cells (DCs) in the context of an inflamed lymph node. Lymph node activation fosters DC and T cell encounters and subsequently provides newly primed T cells with nurturing conditions. We dissected these two aspects by infusing in vitro primed CD8 + T cells into naive recipient mice harboring a single activated lymph node and comparing the fate of these T cells with those infused into control recipients. Brief (20 h) in vitro priming empowered the T cells to expand in vivo without further Ag stimulation. This primary response was not affected by the presence or absence of a nonspecifically activated lymph node. In contrast, in vivo antigenic challenge after contraction of the primary response resulted in significantly stronger secondary T cell responses in mice harboring activated lymph nodes, demonstrating that the availability of an activated lymph node supported the generation of T cell memory in an Ag-unrelated manner. The presence of an activated lymph node during the expansion and contraction phase of the primary response did not endow T cells with an instructional program for increased survival or secondary expansion, but primarily served to conserve increased numbers of T cells. Copyright © 2010 by The American Association of Immunologists, Inc.
AB - Priming of naive CD8+ T cells by pathogens or vaccines generally involves their interaction with Ag-loaded dendritic cells (DCs) in the context of an inflamed lymph node. Lymph node activation fosters DC and T cell encounters and subsequently provides newly primed T cells with nurturing conditions. We dissected these two aspects by infusing in vitro primed CD8 + T cells into naive recipient mice harboring a single activated lymph node and comparing the fate of these T cells with those infused into control recipients. Brief (20 h) in vitro priming empowered the T cells to expand in vivo without further Ag stimulation. This primary response was not affected by the presence or absence of a nonspecifically activated lymph node. In contrast, in vivo antigenic challenge after contraction of the primary response resulted in significantly stronger secondary T cell responses in mice harboring activated lymph nodes, demonstrating that the availability of an activated lymph node supported the generation of T cell memory in an Ag-unrelated manner. The presence of an activated lymph node during the expansion and contraction phase of the primary response did not endow T cells with an instructional program for increased survival or secondary expansion, but primarily served to conserve increased numbers of T cells. Copyright © 2010 by The American Association of Immunologists, Inc.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649901105&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/20696863
U2 - https://doi.org/10.4049/jimmunol.0904046
DO - https://doi.org/10.4049/jimmunol.0904046
M3 - Article
C2 - 20696863
SN - 0022-1767
VL - 185
SP - 3167
EP - 3173
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -