Sequence determinants as key regulators in gene expression of T cells

Benoit P. Nicolet, Nordin D. Zandhuis, V. Maria Lattanzio, Monika C. Wolkers

Research output: Contribution to JournalReview articleAcademicpeer-review

3 Citations (Scopus)

Abstract

T cell homeostasis, T cell differentiation, and T cell effector function rely on the constant fine-tuning of gene expression. To alter the T cell state, substantial remodeling of the proteome is required. This remodeling depends on the intricate interplay of regulatory mechanisms, including post-transcriptional gene regulation. In this review, we discuss how the sequence of a transcript influences these post-transcriptional events. In particular, we review how sequence determinants such as sequence conservation, GC content, and chemical modifications define the levels of the mRNA and the protein in a T cell. We describe the effect of different forms of alternative splicing on mRNA expression and protein production, and their effect on subcellular localization. In addition, we discuss the role of sequences and structures as binding hubs for miRNAs and RNA-binding proteins in T cells. The review thus highlights how the intimate interplay of post-transcriptional mechanisms dictate cellular fate decisions in T cells.
Original languageEnglish
Pages (from-to)10-29
Number of pages20
JournalImmunological Reviews
Volume304
Issue number1
Early online date2021
DOIs
Publication statusPublished - Nov 2021

Keywords

  • GC content
  • RNA binding
  • T cell
  • codon usage
  • miRNA
  • sequence

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