TY - JOUR
T1 - Sesquiterpene lactones of Moquiniastrum polymorphum subsp. floccosum have antineoplastic effects in Walker-256 tumor-bearing rats
AU - Martins, Gracianny Gomes
AU - Lívero, Francislaine Aparecida Dos Reis
AU - Stolf, Aline Maria
AU - Kopruszinski, Caroline Machado
AU - Cardoso, Cibele Campos
AU - Beltrame, Olair Carlos
AU - Queiroz-Telles, José Ederaldo
AU - Strapasson, Regiane Lauriano Batista
AU - Stefanello, Maria Élida Alves
AU - Oude-Elferink, Ronald
AU - Acco, Alexandra
PY - 2015/2/25
Y1 - 2015/2/25
N2 - Background and aim: This study aimed to evaluate the in vivo antitumor actions and toxicity of the dichloromethane fraction (F1B) of Moquiniastrum polymorphum subsp. floccosum (formerly Gochnatia polymorpha ssp. floccosa), composed of sesquiterpene lactones, against Walker-256 carcinosarcoma in rats. Methods: Male Wistar rats received 100 mg kg-1 F1B per day orally for 16 days after subcutaneous inoculation of Walker-256 cells in the pelvic limb. The tumor progression was monitored, and after treatment, tumor weight, oxidative stress, plasma biochemistry, inflammatory parameters, gene expression and histology of tumor and/or liver were evaluated. The toxicity of F1B was analyzed through the relative weight of organs. Additionally, an LD50 test was performed in mice. Results: F1B treatment significantly reduced tumor volume and weight. There was no difference in oxidative stress in tumor tissue after treatment. F1B treatment modified hepatic glutathione and superoxide dismutase, and normalized plasma glucose, alkaline phosphatase, and amylase. F1B did not affect the activity of myeloperoxidase and N-acetylglucosaminidase or the nitric oxide levels in tumor tissue. However, F1B decreased the tumor necrosis factor (TNF)-α levels. Additionally, F1B increased apoptosis in the tumor, mediated by up-regulation of the p53 and Bax gene expression. No clinical signs of toxicity or death were observed in the rats treated with F1B. The LD50 calculated for mice was 1209 mg kg-1. Conclusions: F1B, which is rich in sesquiterpene lactones, showed antitumor activity against Walker-256 carcinosarcoma. This effect may be, at least in part, related to the induction of apoptosis and inhibition of TNF-α signaling.
AB - Background and aim: This study aimed to evaluate the in vivo antitumor actions and toxicity of the dichloromethane fraction (F1B) of Moquiniastrum polymorphum subsp. floccosum (formerly Gochnatia polymorpha ssp. floccosa), composed of sesquiterpene lactones, against Walker-256 carcinosarcoma in rats. Methods: Male Wistar rats received 100 mg kg-1 F1B per day orally for 16 days after subcutaneous inoculation of Walker-256 cells in the pelvic limb. The tumor progression was monitored, and after treatment, tumor weight, oxidative stress, plasma biochemistry, inflammatory parameters, gene expression and histology of tumor and/or liver were evaluated. The toxicity of F1B was analyzed through the relative weight of organs. Additionally, an LD50 test was performed in mice. Results: F1B treatment significantly reduced tumor volume and weight. There was no difference in oxidative stress in tumor tissue after treatment. F1B treatment modified hepatic glutathione and superoxide dismutase, and normalized plasma glucose, alkaline phosphatase, and amylase. F1B did not affect the activity of myeloperoxidase and N-acetylglucosaminidase or the nitric oxide levels in tumor tissue. However, F1B decreased the tumor necrosis factor (TNF)-α levels. Additionally, F1B increased apoptosis in the tumor, mediated by up-regulation of the p53 and Bax gene expression. No clinical signs of toxicity or death were observed in the rats treated with F1B. The LD50 calculated for mice was 1209 mg kg-1. Conclusions: F1B, which is rich in sesquiterpene lactones, showed antitumor activity against Walker-256 carcinosarcoma. This effect may be, at least in part, related to the induction of apoptosis and inhibition of TNF-α signaling.
KW - Acute toxicity (LD)
KW - Cambará
KW - Cancer
KW - Gochnatia polymorpha ssp. floccosa
KW - Moquiniastrum polymorphum subsp. floccosum
KW - Walker-256 tumor
UR - http://www.scopus.com/inward/record.url?scp=84921987639&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.cbi.2015.01.018
DO - https://doi.org/10.1016/j.cbi.2015.01.018
M3 - Article
C2 - 25616030
SN - 0009-2797
VL - 228
SP - 46
EP - 56
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
ER -