TY - JOUR
T1 - SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells
AU - Murakami-Tonami, Yuko
AU - Ikeda, Haruna
AU - Yamagishi, Ryota
AU - Inayoshi, Mao
AU - Inagaki, Shiho
AU - Kishida, Satoshi
AU - Komata, Yosuke
AU - Koster, Jan
AU - Takeuchi, Ichiro
AU - Kondo, Yutaka
AU - Maeda, Tohru
AU - Sekido, Yoshitaka
AU - Murakami, Hiroshi
AU - Kadomatsu, Kenji
PY - 2016
Y1 - 2016
N2 - Shugoshin 1 (SGO1) is required for accurate chromosome segregation during mitosis and meiosis; however, its other functions, especially at interphase, are not clearly understood. Here, we found that downregulation of SGO1 caused a synergistic phenotype in cells overexpressing MYCN. Downregulation of SGO1 impaired proliferation and induced DNA damage followed by a senescence-like phenotype only in MYCN-overexpressing neuroblastoma cells. In these cells, SGO1 knockdown induced DNA damage, even during interphase, and this effect was independent of cohesin. Furthermore, MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. Therefore, we propose that SGO1 represents a potential molecular target for treatment of MYCN-amplified neuroblastoma
AB - Shugoshin 1 (SGO1) is required for accurate chromosome segregation during mitosis and meiosis; however, its other functions, especially at interphase, are not clearly understood. Here, we found that downregulation of SGO1 caused a synergistic phenotype in cells overexpressing MYCN. Downregulation of SGO1 impaired proliferation and induced DNA damage followed by a senescence-like phenotype only in MYCN-overexpressing neuroblastoma cells. In these cells, SGO1 knockdown induced DNA damage, even during interphase, and this effect was independent of cohesin. Furthermore, MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. Therefore, we propose that SGO1 represents a potential molecular target for treatment of MYCN-amplified neuroblastoma
U2 - https://doi.org/10.1038/srep31615
DO - https://doi.org/10.1038/srep31615
M3 - Article
C2 - 27539729
SN - 2045-2322
VL - 6
SP - 31615
JO - Scientific reports
JF - Scientific reports
ER -