Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

Claire M. Mugasa; Thierry Laurent; Gerard J. Schoone; Frank L. Basiye; Alfarazdeg A. Saad; Sayda El Safi; Piet A. Kager; Henk Dfh Schallig

doi:https://doi.org/10.1186/1756-3305-3-13

Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

Claire M. Mugasa, Thierry Laurent, Gerard J. Schoone, Frank L. Basiye, Alfarazdeg A. Saad, Sayda El Safi, Piet A. Kager, Henk Dfh Schallig

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

Abstract

ABSTRACT: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n=30) and cutaneous leishmaniasis from South America (n=70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer

Original language	English
Pages (from-to)	13
Journal	Parasites & vectors
Volume	3
Issue number	1
DOIs	https://doi.org/10.1186/1756-3305-3-13
Publication status	Published - 2010

Access to Document

https://doi.org/10.1186/1756-3305-3-13

Cite this

@article{5a1592b164d54cd49810e5e4d2eafe2b,

title = "Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis",

abstract = "ABSTRACT: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n=30) and cutaneous leishmaniasis from South America (n=70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer",

author = "Mugasa, {Claire M.} and Thierry Laurent and Schoone, {Gerard J.} and Basiye, {Frank L.} and Saad, {Alfarazdeg A.} and {El Safi}, Sayda and Kager, {Piet A.} and Schallig, {Henk Dfh}",

year = "2010",

doi = "https://doi.org/10.1186/1756-3305-3-13",

language = "English",

volume = "3",

pages = "13",

journal = "Parasites & vectors",

issn = "1756-3305",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

AU - Mugasa, Claire M.

AU - Laurent, Thierry

AU - Schoone, Gerard J.

AU - Basiye, Frank L.

AU - Saad, Alfarazdeg A.

AU - El Safi, Sayda

AU - Kager, Piet A.

AU - Schallig, Henk Dfh

PY - 2010

Y1 - 2010

N2 - ABSTRACT: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n=30) and cutaneous leishmaniasis from South America (n=70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer

AB - ABSTRACT: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n=30) and cutaneous leishmaniasis from South America (n=70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer

U2 - https://doi.org/10.1186/1756-3305-3-13

DO - https://doi.org/10.1186/1756-3305-3-13

M3 - Article

C2 - 20196849

SN - 1756-3305

VL - 3

SP - 13

JO - Parasites & vectors

JF - Parasites & vectors

IS - 1

ER -