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Abstract

To accurately determine sample optical properties using single fiber reflectance spectroscopy (SFR), an absolute calibration of the reflectance is required. We investigated two SFR calibration methods, using a calibrated mirror and using the Fresnel reflection at the fiber tip as a reference. We compared these to commonly used calibration methods, using either Intralipid-20% in combination with Monte Carlo simulations or Spectralon as a reference. The Fresnel reflection method demonstrated the best reproducibility and yielded the most reliable result. We therefore recommend the Fresnel reflection method for the measured absolute reflectance calibration of SFR.
Original languageEnglish
Article number100502
JournalJournal of biomedical optics
Volume22
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • calibration
  • measured absolute reflectance
  • single-fiber reflectance spectroscopy

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