Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

A. G. Koek; S. M. Bruisten; M. Dierdorp; A. P. van Dam; K. Templeton

doi:https://doi.org/10.1111/j.1469-0691.2006.01566.x

Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

A. G. Koek, S. M. Bruisten, M. Dierdorp, A. P. van Dam, K. Templeton

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivities and specificities of 94-100% were achieved using two real-time PCR platforms, the Rotor-Gene and the iCycler. The assay can be completed within 2 h, enabling reporting in <8 h. This fast and robust assay is suitable for implementation in routine laboratories for diagnosing primary syphilis

Original language	English
Pages (from-to)	1233-1236
Journal	Clinical Microbiology and Infection
Volume	12
Issue number	12
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01566.x
Publication status	Published - 2006

Access to Document

https://doi.org/10.1111/j.1469-0691.2006.01566.x

Cite this

@article{92dd5e76c2f2406b8172a2f0d1febc63,

title = "Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum",

abstract = "A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivities and specificities of 94-100% were achieved using two real-time PCR platforms, the Rotor-Gene and the iCycler. The assay can be completed within 2 h, enabling reporting in <8 h. This fast and robust assay is suitable for implementation in routine laboratories for diagnosing primary syphilis",

author = "Koek, {A. G.} and Bruisten, {S. M.} and M. Dierdorp and {van Dam}, {A. P.} and K. Templeton",

year = "2006",

doi = "https://doi.org/10.1111/j.1469-0691.2006.01566.x",

language = "English",

volume = "12",

pages = "1233--1236",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier Limited",

number = "12",

}

TY - JOUR

T1 - Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

AU - Koek, A. G.

AU - Bruisten, S. M.

AU - Dierdorp, M.

AU - van Dam, A. P.

AU - Templeton, K.

PY - 2006

Y1 - 2006

N2 - A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivities and specificities of 94-100% were achieved using two real-time PCR platforms, the Rotor-Gene and the iCycler. The assay can be completed within 2 h, enabling reporting in <8 h. This fast and robust assay is suitable for implementation in routine laboratories for diagnosing primary syphilis

AB - A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivities and specificities of 94-100% were achieved using two real-time PCR platforms, the Rotor-Gene and the iCycler. The assay can be completed within 2 h, enabling reporting in <8 h. This fast and robust assay is suitable for implementation in routine laboratories for diagnosing primary syphilis

U2 - https://doi.org/10.1111/j.1469-0691.2006.01566.x

DO - https://doi.org/10.1111/j.1469-0691.2006.01566.x

M3 - Article

C2 - 17121633

SN - 1198-743X

VL - 12

SP - 1233

EP - 1236

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 12

ER -