Structural and functional connectivity reconstruction with CATO - A Connectivity Analysis TOolbox

Siemon C. de Lange, Koen Helwegen, Martijn P. van den Heuvel

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)


We describe a Connectivity Analysis TOolbox (CATO) for the reconstruction of structural and functional brain connectivity based on diffusion weighted imaging and resting-state functional MRI data. CATO is a multimodal software package that enables researchers to run end-to-end reconstructions from MRI data to structural and functional connectome maps, customize their analyses and utilize various software packages to preprocess data. Structural and functional connectome maps can be reconstructed with respect to user-defined (sub)cortical atlases providing aligned connectivity matrices for integrative multimodal analyses. We outline the implementation and usage of the structural and functional processing pipelines in CATO. Performance was calibrated with respect to simulated diffusion weighted imaging data from the ITC2015 challenge and test-retest diffusion weighted imaging data and resting-state functional MRI data from the Human Connectome Project. CATO is open-source software distributed under the MIT License and available as a MATLAB toolbox and as a stand-alone application at

Original languageEnglish
Article number120108
Pages (from-to)1-12
Number of pages12
Early online date12 Apr 2023
Publication statusPublished - 1 Jun 2023

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