TY - JOUR
T1 - Structure and evolution of the human SPRR3 gene
T2 - Implications for function and regulation
AU - Fischer, David F.
AU - Sark, Muriëlle W.J.
AU - Lehtola, Marika M.
AU - Gibbs, Susan
AU - Van De Putte, Pieter
AU - Backendorf, Claude
PY - 1999/1/1
Y1 - 1999/1/1
N2 - SPRR3, a member of the SPRR family of cornified envelope precursor proteins, is expressed in oral and esophageal epithelia, where it is strictly linked to keratinocyte terminal differentiation. This gene is characterized by intragenic duplications that have created the characteristic proline-rich repeats in the coding sequence, an alternative noncoding exon, and a 200-bp polypyrimidine tract in the promoter region. Mutational analysis of the promoter region and transient transfection in normal human keratinocytes showed that in addition to the polypyrimidine tract, multiple regulatory elements are involved in differentiation-specific expression. These elements include a high-affinity Ets binding Site bound by ESE-1, an AP-1 site (TRE) recognized by the Jun/Fos family of transcription factors, and an ATF/CRE bound by Jun/Fos and ATF factors. The repositioning of the SPRR3 Ets binding site during evolution has a major effect on the relative contribution of this site to promoter activity.
AB - SPRR3, a member of the SPRR family of cornified envelope precursor proteins, is expressed in oral and esophageal epithelia, where it is strictly linked to keratinocyte terminal differentiation. This gene is characterized by intragenic duplications that have created the characteristic proline-rich repeats in the coding sequence, an alternative noncoding exon, and a 200-bp polypyrimidine tract in the promoter region. Mutational analysis of the promoter region and transient transfection in normal human keratinocytes showed that in addition to the polypyrimidine tract, multiple regulatory elements are involved in differentiation-specific expression. These elements include a high-affinity Ets binding Site bound by ESE-1, an AP-1 site (TRE) recognized by the Jun/Fos family of transcription factors, and an ATF/CRE bound by Jun/Fos and ATF factors. The repositioning of the SPRR3 Ets binding site during evolution has a major effect on the relative contribution of this site to promoter activity.
UR - http://www.scopus.com/inward/record.url?scp=0032912588&partnerID=8YFLogxK
U2 - https://doi.org/10.1006/geno.1998.5622
DO - https://doi.org/10.1006/geno.1998.5622
M3 - Article
C2 - 9889002
SN - 0888-7543
VL - 55
SP - 88
EP - 99
JO - Genomics
JF - Genomics
IS - 1
ER -