@article{6473f0151ed94f8f871aa5a8c04742e4,
title = "SUMOylation does not affect cardiac troponin I stability but alters indirectly the development of force in response to Ca2+",
abstract = "Post-translational modification of the myofilament protein troponin I by phosphorylation is known to trigger functional changes that support enhanced contraction and relaxation of the heart. We report for the first time that human troponin I can also be modified by SUMOylation at lysine 177. Functionally, TnI SUMOylation is not a factor in the development of passive and maximal force generation in response to calcium, however this modification seems to act indirectly by preventing SUMOylation of other myofilament proteins to alter calcium sensitivity and cooperativity of myofilaments. Utilising a novel, custom SUMO site-specific antibody that recognises only the SUMOylated form of troponin I, we verify that this modification occurs in human heart and that it is upregulated during disease.",
keywords = "SUMO, heart failure, myocytes, myofilaments, troponin I",
author = "Bracy Fertig and Jiayue Ling and Nollet, {Edgar E.} and Sara Dobi and Tara Busiau and Kiyotake Ishikawa and Kelly Yamada and Ahyoung Lee and Changwon Kho and Lauren Wills and Tibbo, {Amy J.} and Mark Scott and Kirsten Grant and Campbell, {Kenneth S.} and Birks, {Emma J.} and Niall MacQuaide and Roger Hajjar and Smith, {Godfrey L.} and {van der Velden}, Jolanda and Baillie, {George S.}",
note = "Funding Information: This work was funded by the BHF PhD studentship programme U.K. to B.F. and G.S.B. BHF Grant FS/15/64/32035. We acknowledge the patients and families of organ donors who donated cardiac samples. Funding: American Heart Association TP135689, NIH HL148785, University of Kentucky Myocardial Recovery Alliance. We thank Dr Gonzalo S. Tejeda for constructing the graphical abstract. Funding Information: This work was funded by the BHF PhD studentship programme U.K. to B.F. and G.S.B. BHF Grant FS/15/64/32035. We acknowledge the patients and families of organ donors who donated cardiac samples. Funding: American Heart Association TP135689, NIH HL148785, University of Kentucky Myocardial Recovery Alliance. We thank Dr Gonzalo S. Tejeda for constructing the graphical abstract. Publisher Copyright: {\textcopyright} 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.",
year = "2022",
month = oct,
doi = "https://doi.org/10.1111/febs.16537",
language = "English",
volume = "289",
pages = "6267--6285",
journal = "FEBS journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "20",
}