TY - JOUR
T1 - Suppression of rat cytomegalovirus replication by antibodies against gamma interferon
AU - Haagmans, Bart L.
AU - Van Der Meide, Peter H.
AU - Stals, Frans S.
AU - Van Den Eertwegh, Alfons J.M.
AU - Claassen, Eric
AU - Bruggeman, Cathrien A.
AU - Horzinek, Marian C.
AU - Schijns, Virgil E.C.J.
PY - 1994/4
Y1 - 1994/4
N2 - The role of gamma interferon (IFN-γ) in the resolution of rat cytomegalovirus (RCMV) infection was investigated. In the spleen, IFN-γ- producing cells reached maximum numbers on day 7 after infection. Prophylactic treatment with high doses of recombinant rat IFN-γ exerted antiviral activity in fibroblasts and protected immunosuppressed rats against a lethal RCMV challenge. Remarkably, in immunocompetent rats, neutralization of endogenous IFN-γ activity significantly reduced the numbers of RCMV antigen-expressing cells in the spleen, the predominant site of viral replication. Moreover, protection of radiation-immunosuppressed infected rats by transferred immune T cells was enhanced by coinjection of IFN-γ neutralizing antibodies. The observations were paralleled by in vitro findings: low concentrations of IFN-γ enhanced viral replication in both macrophages and fibroblasts. These data suggest that IFN-γ can play different and even opposite roles in the regulation of RCMV replication in vivo; T lymphocytes may contribute to the progression of RCMV infection by secreting IFN-γ.
AB - The role of gamma interferon (IFN-γ) in the resolution of rat cytomegalovirus (RCMV) infection was investigated. In the spleen, IFN-γ- producing cells reached maximum numbers on day 7 after infection. Prophylactic treatment with high doses of recombinant rat IFN-γ exerted antiviral activity in fibroblasts and protected immunosuppressed rats against a lethal RCMV challenge. Remarkably, in immunocompetent rats, neutralization of endogenous IFN-γ activity significantly reduced the numbers of RCMV antigen-expressing cells in the spleen, the predominant site of viral replication. Moreover, protection of radiation-immunosuppressed infected rats by transferred immune T cells was enhanced by coinjection of IFN-γ neutralizing antibodies. The observations were paralleled by in vitro findings: low concentrations of IFN-γ enhanced viral replication in both macrophages and fibroblasts. These data suggest that IFN-γ can play different and even opposite roles in the regulation of RCMV replication in vivo; T lymphocytes may contribute to the progression of RCMV infection by secreting IFN-γ.
UR - http://www.scopus.com/inward/record.url?scp=0028209820&partnerID=8YFLogxK
U2 - https://doi.org/10.1128/jvi.68.4.2305-2312.1994
DO - https://doi.org/10.1128/jvi.68.4.2305-2312.1994
M3 - Article
C2 - 8139015
SN - 0022-538X
VL - 68
SP - 2305
EP - 2312
JO - Journal of Virology
JF - Journal of Virology
IS - 4
ER -