T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED

Chih-Hsi Scott Kuo; Stelios Pavlidis; Matthew Loza; Fred Baribaud; Anthony Rowe; Iaonnis Pandis; Ana Sousa; Julie Corfield; Ratko Djukanovic; Rene Lutter; Peter J. Sterk; Charles Auffray; Yike Guo; Ian M. Adcock; Kian Fan Chung

doi:https://doi.org/10.1183/13993003.02135-2016

T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED

Chih-Hsi Scott Kuo, Stelios Pavlidis, Matthew Loza, Fred Baribaud, Anthony Rowe, Iaonnis Pandis, Ana Sousa, Julie Corfield, Ratko Djukanovic, Rene Lutter, Peter J. Sterk, Charles Auffray, Yike Guo, Ian M. Adcock, Kian Fan Chung

Research output: Contribution to journal › Article › Academic › peer-review

285 Citations (Scopus)

Abstract

TSLPR), TAC2 (characterised by interferon-, tumour necrosis factor-α- and inflammasome-associated genes) and TAC3 (characterised by genes of metabolic pathways, ubiquitination and mitochondrial function). TAC1 showed the highest enrichment of gene signatures for interleukin-13/T-helper cell type 2 (Th2) and innate lymphoid cell type 2. TAC1 had the highest sputum eosinophilia and exhaled nitric oxide fraction, and was restricted to severe asthma with oral corticosteroid dependency, frequent exacerbations and severe airflow obstruction. TAC2 showed the highest sputum neutrophilia, serum C-reactive protein levels and prevalence of eczema. TAC3 had normal to moderately high sputum eosinophils and better preserved forced expiratory volume in 1 s. Gene-protein coexpression networks from TAC1 and TAC2 extended this molecular classification.We defined one Th2-high eosinophilic phenotype TAC1, and two non-Th2 phenotypes TAC2 and TAC3, characterised by inflammasome-associated and metabolic/mitochondrial pathways, respectively

Original language	English
Article number	1602135
Journal	European respiratory journal
Volume	49
Issue number	2
DOIs	https://doi.org/10.1183/13993003.02135-2016
Publication status	Published - 2017

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https://doi.org/10.1183/13993003.02135-2016

Cite this

Kuo, C.-H. S., Pavlidis, S., Loza, M., Baribaud, F., Rowe, A., Pandis, I., Sousa, A., Corfield, J., Djukanovic, R., Lutter, R., Sterk, P. J., Auffray, C., Guo, Y., Adcock, I. M., & Chung, K. F. (2017). T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED. European respiratory journal, 49(2), Article 1602135. https://doi.org/10.1183/13993003.02135-2016

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title = "T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED",

abstract = "TSLPR), TAC2 (characterised by interferon-, tumour necrosis factor-α- and inflammasome-associated genes) and TAC3 (characterised by genes of metabolic pathways, ubiquitination and mitochondrial function). TAC1 showed the highest enrichment of gene signatures for interleukin-13/T-helper cell type 2 (Th2) and innate lymphoid cell type 2. TAC1 had the highest sputum eosinophilia and exhaled nitric oxide fraction, and was restricted to severe asthma with oral corticosteroid dependency, frequent exacerbations and severe airflow obstruction. TAC2 showed the highest sputum neutrophilia, serum C-reactive protein levels and prevalence of eczema. TAC3 had normal to moderately high sputum eosinophils and better preserved forced expiratory volume in 1 s. Gene-protein coexpression networks from TAC1 and TAC2 extended this molecular classification.We defined one Th2-high eosinophilic phenotype TAC1, and two non-Th2 phenotypes TAC2 and TAC3, characterised by inflammasome-associated and metabolic/mitochondrial pathways, respectively",

author = "Kuo, {Chih-Hsi Scott} and Stelios Pavlidis and Matthew Loza and Fred Baribaud and Anthony Rowe and Iaonnis Pandis and Ana Sousa and Julie Corfield and Ratko Djukanovic and Rene Lutter and Sterk, {Peter J.} and Charles Auffray and Yike Guo and Adcock, {Ian M.} and Chung, {Kian Fan}",

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doi = "https://doi.org/10.1183/13993003.02135-2016",

language = "English",

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journal = "European respiratory journal",

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Kuo, C-HS, Pavlidis, S, Loza, M, Baribaud, F, Rowe, A, Pandis, I, Sousa, A, Corfield, J, Djukanovic, R, Lutter, R , Sterk, PJ, Auffray, C, Guo, Y, Adcock, IM & Chung, KF 2017, 'T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED', European respiratory journal, vol. 49, no. 2, 1602135. https://doi.org/10.1183/13993003.02135-2016

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T1 - T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED

AU - Kuo, Chih-Hsi Scott

AU - Pavlidis, Stelios

AU - Loza, Matthew

AU - Baribaud, Fred

AU - Rowe, Anthony

AU - Pandis, Iaonnis

AU - Sousa, Ana

AU - Corfield, Julie

AU - Djukanovic, Ratko

AU - Lutter, Rene

AU - Sterk, Peter J.

AU - Auffray, Charles

AU - Guo, Yike

AU - Adcock, Ian M.

AU - Chung, Kian Fan

PY - 2017

Y1 - 2017

N2 - TSLPR), TAC2 (characterised by interferon-, tumour necrosis factor-α- and inflammasome-associated genes) and TAC3 (characterised by genes of metabolic pathways, ubiquitination and mitochondrial function). TAC1 showed the highest enrichment of gene signatures for interleukin-13/T-helper cell type 2 (Th2) and innate lymphoid cell type 2. TAC1 had the highest sputum eosinophilia and exhaled nitric oxide fraction, and was restricted to severe asthma with oral corticosteroid dependency, frequent exacerbations and severe airflow obstruction. TAC2 showed the highest sputum neutrophilia, serum C-reactive protein levels and prevalence of eczema. TAC3 had normal to moderately high sputum eosinophils and better preserved forced expiratory volume in 1 s. Gene-protein coexpression networks from TAC1 and TAC2 extended this molecular classification.We defined one Th2-high eosinophilic phenotype TAC1, and two non-Th2 phenotypes TAC2 and TAC3, characterised by inflammasome-associated and metabolic/mitochondrial pathways, respectively

AB - TSLPR), TAC2 (characterised by interferon-, tumour necrosis factor-α- and inflammasome-associated genes) and TAC3 (characterised by genes of metabolic pathways, ubiquitination and mitochondrial function). TAC1 showed the highest enrichment of gene signatures for interleukin-13/T-helper cell type 2 (Th2) and innate lymphoid cell type 2. TAC1 had the highest sputum eosinophilia and exhaled nitric oxide fraction, and was restricted to severe asthma with oral corticosteroid dependency, frequent exacerbations and severe airflow obstruction. TAC2 showed the highest sputum neutrophilia, serum C-reactive protein levels and prevalence of eczema. TAC3 had normal to moderately high sputum eosinophils and better preserved forced expiratory volume in 1 s. Gene-protein coexpression networks from TAC1 and TAC2 extended this molecular classification.We defined one Th2-high eosinophilic phenotype TAC1, and two non-Th2 phenotypes TAC2 and TAC3, characterised by inflammasome-associated and metabolic/mitochondrial pathways, respectively

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DO - https://doi.org/10.1183/13993003.02135-2016

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