TY - JOUR
T1 - The association between viral load and concurrent human papillomavirus infection at the genital and anal sites of young women and the impact of vaccination
AU - PHS Fryslân
AU - PHS Twente
AU - PHS Hart voor Brabant
AU - PHS Amsterdam
AU - PHS Gelderland-Midden
AU - van Eer, Kahren
AU - Laâbi, Ihsane
AU - van Benthem, Birgit H. B.
AU - Steenbergen, Renske D. M.
AU - King, Audrey J.
AU - Adema, D.
AU - Buist-Arkema, R.
AU - Beerens, A.
AU - Luijt, D.
AU - Meijer, S.
AU - Schirm, J.
AU - ETZ Hospital Tilburg: A. Buiting
AU - Peeters, M.
AU - Rossen, J.
AU - Verbakel, H.
AU - van Esch, P.
AU - Verweij, J.
AU - Erasmus Medical Center
AU - van der Eijk, A.
AU - Huisman, R.
AU - Kerkhof, C.
AU - Korff, H.
AU - Schutten, M.
AU - Velzing, J.
AU - University Medical Center Utrecht
AU - Verduyn-Lunel, F.
AU - Lakbiach, S.
AU - van Rosmalen, P.
AU - Schuurman, R.
AU - Doorn, E.
AU - Masthoff, L.
AU - Pannekoek, E.
AU - Sigurdsson, V.
AU - Public Health Laboratory Amsterdam
AU - Abma, D.
AU - Adams, K.
AU - Bruisten, S.
AU - Linde, I.
AU - Oostvogel, P.
AU - Touwen, C.
AU - Vermeulen, W.
AU - Maastricht University Medical Center
AU - Brink, A.
AU - Nelissen, J.
AU - Wolffs, P.
AU - Jeroen Bosch Hospital
AU - Duijvendijk, N.
AU - Schneeberger, P.
AU - Radboud University Medical Center
AU - Dinnissen van Poppel, M.
AU - Melchers, W.
AU - Poort, Y.
AU - Izore, M. Hooghiemstra
AU - LabMicTA
AU - Peters, M.
AU - Medical Laboratory dr. Stein and Collegae
AU - Janssen, J.
AU - Canisius Wilhelmina Hospital
AU - Public Health Services: PHS Drenthe
AU - PHS IJsselland
AU - PHS Gelderland-Zuid
AU - PHS Rotterdam-Rijnmond
AU - PHS Groningen
AU - PHS Zuid Limburg
AU - Brouwers, E.
AU - Smit, M.
N1 - Funding Information: This work was supported by the Ministry of Health, Welfare and Sports, the Netherlands . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 The Author(s)
PY - 2022/6/1
Y1 - 2022/6/1
N2 - Concurrent genital-anal human papillomavirus (HPV) infections may impose an increased anal cancer risk in women with HPV-related genital lesions. High viral load may facilitate genital-anal HPV concurrence. Genital and anal HPV is reduced by a bivalent HPV16/18 vaccine, yet the effect on concurrent genital-anal HPV remains unclear. This study analyzed viral load in concurrent genital-anal HPV infections, relative to genital-only and anal-only HPV infections and the impact of vaccination in young women. We included 1074 women, who provided both genital and anal swabs. HPV detection and genotyping was performed using the SPF10-DEIA-LiPA25. HPV copy numbers were measured with type-specific qPCRs and corrected for cellular content to obtain the viral load. Concurrent genital-anal HPV often had significantly higher genital viral load (0.09–371 c/cell) than genital-only HPV (3.17E-04-15.9 c/cell, p < 0.0001 to p < 0.05). Moreover, nearly all concurrent genital-anal HPV types had higher genital copy numbers per PCR reaction (157-416E04 c/rxn) than anal copy numbers (0.90–884E01 c/rxn, p < 0.0001 to p < 0.001). Vaccinated women had significantly less infections with HPV16/18 vaccine-types (2.8% vs 13.7%, p < 0.0001) and HPV31/35/45 cross-protective types (7.4% vs 21.1%, p < 0.0001) than unvaccinated women. In conclusion, particularly high genital viral load is found in concurrent genital-anal HPV infections, which are effectively reduced by vaccination.
AB - Concurrent genital-anal human papillomavirus (HPV) infections may impose an increased anal cancer risk in women with HPV-related genital lesions. High viral load may facilitate genital-anal HPV concurrence. Genital and anal HPV is reduced by a bivalent HPV16/18 vaccine, yet the effect on concurrent genital-anal HPV remains unclear. This study analyzed viral load in concurrent genital-anal HPV infections, relative to genital-only and anal-only HPV infections and the impact of vaccination in young women. We included 1074 women, who provided both genital and anal swabs. HPV detection and genotyping was performed using the SPF10-DEIA-LiPA25. HPV copy numbers were measured with type-specific qPCRs and corrected for cellular content to obtain the viral load. Concurrent genital-anal HPV often had significantly higher genital viral load (0.09–371 c/cell) than genital-only HPV (3.17E-04-15.9 c/cell, p < 0.0001 to p < 0.05). Moreover, nearly all concurrent genital-anal HPV types had higher genital copy numbers per PCR reaction (157-416E04 c/rxn) than anal copy numbers (0.90–884E01 c/rxn, p < 0.0001 to p < 0.001). Vaccinated women had significantly less infections with HPV16/18 vaccine-types (2.8% vs 13.7%, p < 0.0001) and HPV31/35/45 cross-protective types (7.4% vs 21.1%, p < 0.0001) than unvaccinated women. In conclusion, particularly high genital viral load is found in concurrent genital-anal HPV infections, which are effectively reduced by vaccination.
KW - Anal
KW - Concurrent
KW - Genital
KW - Human papillomavirus
KW - Vaccination
KW - Viral load
UR - http://www.scopus.com/inward/record.url?scp=85123246096&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.tvr.2021.200233
DO - https://doi.org/10.1016/j.tvr.2021.200233
M3 - Article
C2 - 34958987
SN - 2666-6790
VL - 13
JO - Tumour virus research
JF - Tumour virus research
M1 - 200233
ER -