TY - JOUR
T1 - The functional microscopic neuroanatomy of the human subthalamic nucleus
AU - Alkemade, A.
AU - de Hollander, G.
AU - Miletic, S.
AU - Keuken, M.C.
AU - Balesar, R.
AU - de Boer, O.
AU - Swaab, D.F.
AU - Forstmann, B.U.
N1 - With supplementary file.
PY - 2019/12
Y1 - 2019/12
N2 - The subthalamic nucleus (STN) is successfully used as a surgical target for deep brain stimulation in the treatment of movement disorders. Interestingly, the internal structure of the STN is still incompletely understood. The objective of the present study was to investigate three-dimensional (3D) immunoreactivity patterns for 12 individual protein markers for GABA-ergic, serotonergic, dopaminergic as well as glutamatergic signaling. We analyzed the immunoreactivity using optical densities and created a 3D reconstruction of seven postmortem human STNs. Quantitative modeling of the reconstructed 3D immunoreactivity patterns revealed that the applied protein markers show a gradient distribution in the STN. These gradients were predominantly organized along the ventromedial to dorsolateral axis of the STN. The results are of particular interest in view of the theoretical underpinning for surgical targeting, which is based on a tripartite distribution of cognitive, limbic and motor function in the STN.
AB - The subthalamic nucleus (STN) is successfully used as a surgical target for deep brain stimulation in the treatment of movement disorders. Interestingly, the internal structure of the STN is still incompletely understood. The objective of the present study was to investigate three-dimensional (3D) immunoreactivity patterns for 12 individual protein markers for GABA-ergic, serotonergic, dopaminergic as well as glutamatergic signaling. We analyzed the immunoreactivity using optical densities and created a 3D reconstruction of seven postmortem human STNs. Quantitative modeling of the reconstructed 3D immunoreactivity patterns revealed that the applied protein markers show a gradient distribution in the STN. These gradients were predominantly organized along the ventromedial to dorsolateral axis of the STN. The results are of particular interest in view of the theoretical underpinning for surgical targeting, which is based on a tripartite distribution of cognitive, limbic and motor function in the STN.
KW - Basal ganglia
KW - Immunocytochemistry
KW - Neuroanatomy
KW - Subthalamic nucleus
UR - http://www.scopus.com/inward/record.url?scp=85074040876&partnerID=8YFLogxK
UR - https://pure.uva.nl/ws/files/43145080/429_2019_1960_MOESM1_ESM.pdf
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074040876&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31562531
U2 - https://doi.org/10.1007/s00429-019-01960-3
DO - https://doi.org/10.1007/s00429-019-01960-3
M3 - Article
C2 - 31562531
SN - 1863-2653
VL - 224
SP - 3212
EP - 3227
JO - Brain Structure and Function
JF - Brain Structure and Function
IS - 9
ER -