The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end

G. J. van Ommen; A. C. Arnberg; F. Baas; H. Brocas; A. Sterk; W. H. Tegelaers; G. Vassart; J. J. de Vijlder

The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end

G. J. van Ommen, A. C. Arnberg, F. Baas, H. Brocas, A. Sterk, W. H. Tegelaers, G. Vassart, J. J. de Vijlder

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Abstract

We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences

Original language	English
Pages (from-to)	2273-2285
Journal	Nucleic Acids Research
Volume	11
Issue number	8
Publication status	Published - 1983

Cite this

@article{6fac96da99f448c4aa6198088db677f5,

title = "The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end",

abstract = "We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences",

author = "{van Ommen}, {G. J.} and Arnberg, {A. C.} and F. Baas and H. Brocas and A. Sterk and Tegelaers, {W. H.} and G. Vassart and {de Vijlder}, {J. J.}",

year = "1983",

language = "English",

volume = "11",

pages = "2273--2285",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "8",

}

TY - JOUR

T1 - The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end

AU - van Ommen, G. J.

AU - Arnberg, A. C.

AU - Baas, F.

AU - Brocas, H.

AU - Sterk, A.

AU - Tegelaers, W. H.

AU - Vassart, G.

AU - de Vijlder, J. J.

PY - 1983

Y1 - 1983

N2 - We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences

AB - We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130-210 nucleotides, split by introns of 1 to 15-17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thus far in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences

M3 - Article

C2 - 6304645

SN - 0305-1048

VL - 11

SP - 2273

EP - 2285

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 8

ER -