TY - JOUR
T1 - The influence of nifedipine and mioflazine on mitochondrial calcium overload in normoxic and ischaemic guinea-pig hearts
AU - Hugtenburg, Jacqueline G.
AU - Van Voorst, Mario J.D.
AU - Van Marle, Jan
AU - Mathy, Marie Jeanne
AU - Beckeringh, Jan Jacob
AU - Van Veen, Henk A.
AU - Hendriks, Maarten G.C.
AU - Van Zwieten, Pieter A.
PY - 1990/3/13
Y1 - 1990/3/13
N2 - The influence of nifedipine (20 nM) and mioflazine (300 nM), i.e. concentrations inducing a 60-70% recovery of cardiac function during reperfusion of globally ischaemic guinea-pig working beans, on the mitochondrial calcium content was investigated in normoxic, globally ischaemic and reperfused globally ischaemic guinea-pig working hearts. Mitochondrial calcium was determined electronmicroscopically with the oxalate-pyroantimonate method. In normoxic hearts both nifedipine and mioflazine reduced the mitochondrial calcium content. Global ischaemia for 45 min and subsequent reperfusion for 25 min resulted in a pronounced mitochondrial calcium overload and damage to the cellular structure. In ischaemic and in reperfusion hearts the drugs maintained mitochondrial calcium at pre-ischaemic levels and decreased the damage to the cellular structure.
AB - The influence of nifedipine (20 nM) and mioflazine (300 nM), i.e. concentrations inducing a 60-70% recovery of cardiac function during reperfusion of globally ischaemic guinea-pig working beans, on the mitochondrial calcium content was investigated in normoxic, globally ischaemic and reperfused globally ischaemic guinea-pig working hearts. Mitochondrial calcium was determined electronmicroscopically with the oxalate-pyroantimonate method. In normoxic hearts both nifedipine and mioflazine reduced the mitochondrial calcium content. Global ischaemia for 45 min and subsequent reperfusion for 25 min resulted in a pronounced mitochondrial calcium overload and damage to the cellular structure. In ischaemic and in reperfusion hearts the drugs maintained mitochondrial calcium at pre-ischaemic levels and decreased the damage to the cellular structure.
KW - Heart (guinea-pig, working)
KW - Ischaemia (global)
KW - Mioflazine
KW - Mitochondrial calcium
KW - Nifedipine
UR - http://www.scopus.com/inward/record.url?scp=0025264849&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0014-2999(90)94794-X
DO - https://doi.org/10.1016/0014-2999(90)94794-X
M3 - Article
C2 - 2332029
SN - 0014-2999
VL - 178
SP - 71
EP - 78
JO - European journal of pharmacology
JF - European journal of pharmacology
IS - 1
ER -