The presence of alpha-catenin in the VE-cadherin complex is required for efficient transendothelial migration of leukocytes

J.D. van Buul, F.P. van Alphen, P.L. Hordijk

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The majority of the leukocytes cross the endothelial lining of the vessels through cell-cell junctions. The junctional protein Vascular Endothelial (VE)-cadherin is transiently re-distributed from sites of cell-cell contacts during passage of leukocytes. VE-cadherin is part of a protein complex comprising p120-catenin and beta-catenin as intracellular partners. Beta-catenin connects VE-cadherin to alpha-catenin. This VE-cadherin-catenin complex is believed to dynamically control endothelial cell-cell junctions and to regulate the passage of leukocytes, although not much is known about the role of alpha-and beta-catenin during the process of transendothelial migration (TEM). In order to study the importance of the interaction between alpha-and beta-catenin in TEM, we used a cell-permeable version of the peptide encoding the binding site of alpha-catenin for beta-catenin (S27D). The data show that S27D interferes with the interaction between alpha-and beta-catenin and induces a reversible decrease in electrical resistance of the endothelial monolayer. In addition, S27D co-localized with beta-catenin at cell-cell junctions. Surprisingly, transmigration of neutrophils across endothelial monolayers was blocked in the presence of S27D. In conclusion, our results show for the first time that the association of alpha-catenin with the cadherin-catenin complex is required for efficient leukocyte TEM
Original languageUndefined/Unknown
Pages (from-to)695-705
Number of pages11
JournalInternational Journal of Biological Sciences
Issue number7
Publication statusPublished - 1 Jan 2009


  • Catenin
  • Cell-cell junction
  • Permeability
  • Transendothelial migration
  • VE-cadherin

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