The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux

Carlos Vrins; Edwin Vink; Kristin E. Vandenberghe; Raoul Frijters; Jurgen Seppen; Albert K. Groen

doi:https://doi.org/10.1016/j.febslet.2007.08.052

The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux

Carlos Vrins, Edwin Vink, Kristin E. Vandenberghe, Raoul Frijters, Jurgen Seppen, Albert K. Groen

Research output: Contribution to journal › Article › Academic › peer-review

52 Citations (Scopus)

Abstract

The ATP binding cassette transporters ABCG5 and ABCG8 are indispensable for hepatobiliary cholesterol transport. In this study, we investigated the specificity of the heterodimer for cholesterol acceptors. Dog gallbladder epithelial cells were mono- or double-transfected with lentiviral mouse Abcg5 and Abcg8 vectors. Double-transfected cells showed increased efflux to different bile salt (BS) species, while mono-transfected cells did not show enhanced efflux. The efflux was initiated at micellar concentrations and addition of phosphatidylcholine increased efflux. Cholesterol secretion was highly BS dependent, whereas other cholesterol acceptors such as ApoAI, HDL or methyl-β-cyclodextrin did not elicit Abcg5/g8 dependent cholesterol secretion.

Original language	English
Pages (from-to)	4616-4620
Number of pages	5
Journal	FEBS letters
Volume	581
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2007.08.052
Publication status	Published - 2 Oct 2007

Keywords

ABC transporter
ABCG5
ABCG8
Cholesterol
Cholesterol efflux

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https://doi.org/10.1016/j.febslet.2007.08.052

Cite this

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title = "The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux",

abstract = "The ATP binding cassette transporters ABCG5 and ABCG8 are indispensable for hepatobiliary cholesterol transport. In this study, we investigated the specificity of the heterodimer for cholesterol acceptors. Dog gallbladder epithelial cells were mono- or double-transfected with lentiviral mouse Abcg5 and Abcg8 vectors. Double-transfected cells showed increased efflux to different bile salt (BS) species, while mono-transfected cells did not show enhanced efflux. The efflux was initiated at micellar concentrations and addition of phosphatidylcholine increased efflux. Cholesterol secretion was highly BS dependent, whereas other cholesterol acceptors such as ApoAI, HDL or methyl-β-cyclodextrin did not elicit Abcg5/g8 dependent cholesterol secretion.",

keywords = "ABC transporter, ABCG5, ABCG8, Cholesterol, Cholesterol efflux",

author = "Carlos Vrins and Edwin Vink and Vandenberghe, {Kristin E.} and Raoul Frijters and Jurgen Seppen and Groen, {Albert K.}",

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T1 - The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux

AU - Vrins, Carlos

AU - Vink, Edwin

AU - Vandenberghe, Kristin E.

AU - Frijters, Raoul

AU - Seppen, Jurgen

AU - Groen, Albert K.

PY - 2007/10/2

Y1 - 2007/10/2

N2 - The ATP binding cassette transporters ABCG5 and ABCG8 are indispensable for hepatobiliary cholesterol transport. In this study, we investigated the specificity of the heterodimer for cholesterol acceptors. Dog gallbladder epithelial cells were mono- or double-transfected with lentiviral mouse Abcg5 and Abcg8 vectors. Double-transfected cells showed increased efflux to different bile salt (BS) species, while mono-transfected cells did not show enhanced efflux. The efflux was initiated at micellar concentrations and addition of phosphatidylcholine increased efflux. Cholesterol secretion was highly BS dependent, whereas other cholesterol acceptors such as ApoAI, HDL or methyl-β-cyclodextrin did not elicit Abcg5/g8 dependent cholesterol secretion.

AB - The ATP binding cassette transporters ABCG5 and ABCG8 are indispensable for hepatobiliary cholesterol transport. In this study, we investigated the specificity of the heterodimer for cholesterol acceptors. Dog gallbladder epithelial cells were mono- or double-transfected with lentiviral mouse Abcg5 and Abcg8 vectors. Double-transfected cells showed increased efflux to different bile salt (BS) species, while mono-transfected cells did not show enhanced efflux. The efflux was initiated at micellar concentrations and addition of phosphatidylcholine increased efflux. Cholesterol secretion was highly BS dependent, whereas other cholesterol acceptors such as ApoAI, HDL or methyl-β-cyclodextrin did not elicit Abcg5/g8 dependent cholesterol secretion.

KW - ABC transporter

KW - ABCG5

KW - ABCG8

KW - Cholesterol

KW - Cholesterol efflux

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U2 - https://doi.org/10.1016/j.febslet.2007.08.052

DO - https://doi.org/10.1016/j.febslet.2007.08.052

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SN - 0014-5793

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EP - 4620

JO - FEBS letters

JF - FEBS letters

IS - 24

ER -

The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux

Abstract

Keywords

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