The Use of Laser Microdissection and SELDI-TOF MS in Ovarian Cancer Tissue to Identify Protein Profiles

Isabelle Cadron; Toon van Gorp; Frederic Amant; Ignace Vergote; Philippe Moerman; Etienne Waelkens; Anneleen Daemen; Raf van de Plas; Bart de Moor; Robert Zeillinger

The Use of Laser Microdissection and SELDI-TOF MS in Ovarian Cancer Tissue to Identify Protein Profiles

Isabelle Cadron, Toon van Gorp, Frederic Amant, Ignace Vergote, Philippe Moerman, Etienne Waelkens, Anneleen Daemen, Raf van de Plas, Bart de Moor, Robert Zeillinger

Other Departments

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Background: There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities. Materials and Methods: This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles. Results: Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients. Conclusion: This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach

Original language	English
Pages (from-to)	1039-1045
Journal	Anticancer research
Volume	29
Issue number	4
Publication status	Published - 2009

Cite this

@article{7627a1d30e564a4dbe206e982e042899,

title = "The Use of Laser Microdissection and SELDI-TOF MS in Ovarian Cancer Tissue to Identify Protein Profiles",

abstract = "Background: There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities. Materials and Methods: This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles. Results: Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients. Conclusion: This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach",

author = "Isabelle Cadron and {van Gorp}, Toon and Frederic Amant and Ignace Vergote and Philippe Moerman and Etienne Waelkens and Anneleen Daemen and {van de Plas}, Raf and {de Moor}, Bart and Robert Zeillinger",

year = "2009",

language = "English",

volume = "29",

pages = "1039--1045",

journal = "Anticancer research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

}

TY - JOUR

T1 - The Use of Laser Microdissection and SELDI-TOF MS in Ovarian Cancer Tissue to Identify Protein Profiles

AU - Cadron, Isabelle

AU - van Gorp, Toon

AU - Amant, Frederic

AU - Vergote, Ignace

AU - Moerman, Philippe

AU - Waelkens, Etienne

AU - Daemen, Anneleen

AU - van de Plas, Raf

AU - de Moor, Bart

AU - Zeillinger, Robert

PY - 2009

Y1 - 2009

N2 - Background: There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities. Materials and Methods: This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles. Results: Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients. Conclusion: This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach

AB - Background: There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities. Materials and Methods: This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles. Results: Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients. Conclusion: This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach

M3 - Article

C2 - 19414343

SN - 0250-7005

VL - 29

SP - 1039

EP - 1045

JO - Anticancer research

JF - Anticancer research

IS - 4

ER -