TY - JOUR
T1 - Thermoultrasonication, ultraviolet-C irradiation, and high-pressure processing
T2 - Novel techniques to preserve insulin in donor human milk
AU - Mank, Elise
AU - Kontopodi, Eva
AU - Heijboer, Annemieke C.
AU - van Elburg, Ruurd M.
AU - Hettinga, Kasper
AU - van Goudoever, Johannes B.
AU - van Toledo, Letty
N1 - Funding Information: The authors wish to thank the study participants for their contributions. The authors would also like to thank the technicians from the Endocrine Laboratory in the Clinical Chemistry department for performing the insulin measurements. Publisher Copyright: © 2021 The Authors
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Background & aims: Donor human milk (DHM) is recommended as the first alternative for preterm infants if their mother's own milk is not available or if the quantity is not sufficient. The most commonly used technique to eliminate microbial contaminants in DHM is holder pasteurization (HoP). However, the heating process during HoP partially destroys milk bioactive factors such as insulin. Therefore, innovative techniques have been developed as alternatives to HoP. The objective of this study was to determine the effect of HoP, high-temperature–short-time (HTST), thermoultrasonication (TUS), ultraviolet-C irradiation (UV-C), and high-pressure processing (HPP) on the insulin concentration in DHM. Methods: Milk samples from 28 non-diabetic mothers were collected. The milk samples were aliquoted and either left untreated or treated with HoP (62.5 °C; 30 min), HTST (72 °C; 15 s), TUS (60 W; 6 min), UV-C (4863 J/L), or HPP (500 MPa; 5 min). Results: The mean insulin concentration in untreated milk was 79 ± 41 pmol/L. The mean insulin retention rate was 67% for HoP, 78% for HTST, 97% for TUS, 94% for UV-C, and 106% for HPP. The mean insulin concentration in milk treated with HoP was significantly lower compared to untreated milk (p = 0.01). Conclusion: TUS, UV-C, and HPP preserve insulin in DHM. The insulin concentration in DHM is affected to a larger extent by HoP than by HTST. These results indicate that TUS, UV-C, and HPP may serve as alternatives to HoP.
AB - Background & aims: Donor human milk (DHM) is recommended as the first alternative for preterm infants if their mother's own milk is not available or if the quantity is not sufficient. The most commonly used technique to eliminate microbial contaminants in DHM is holder pasteurization (HoP). However, the heating process during HoP partially destroys milk bioactive factors such as insulin. Therefore, innovative techniques have been developed as alternatives to HoP. The objective of this study was to determine the effect of HoP, high-temperature–short-time (HTST), thermoultrasonication (TUS), ultraviolet-C irradiation (UV-C), and high-pressure processing (HPP) on the insulin concentration in DHM. Methods: Milk samples from 28 non-diabetic mothers were collected. The milk samples were aliquoted and either left untreated or treated with HoP (62.5 °C; 30 min), HTST (72 °C; 15 s), TUS (60 W; 6 min), UV-C (4863 J/L), or HPP (500 MPa; 5 min). Results: The mean insulin concentration in untreated milk was 79 ± 41 pmol/L. The mean insulin retention rate was 67% for HoP, 78% for HTST, 97% for TUS, 94% for UV-C, and 106% for HPP. The mean insulin concentration in milk treated with HoP was significantly lower compared to untreated milk (p = 0.01). Conclusion: TUS, UV-C, and HPP preserve insulin in DHM. The insulin concentration in DHM is affected to a larger extent by HoP than by HTST. These results indicate that TUS, UV-C, and HPP may serve as alternatives to HoP.
KW - Breast feeding
KW - Milk bank
KW - Pasteurization
KW - Preterm
KW - Trophic factor
UR - http://www.scopus.com/inward/record.url?scp=85117206620&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.clnu.2021.09.028
DO - https://doi.org/10.1016/j.clnu.2021.09.028
M3 - Article
C2 - 34666256
SN - 0261-5614
VL - 40
SP - 5655
EP - 5658
JO - Clinical Nutrition
JF - Clinical Nutrition
IS - 11
ER -