Three-point repositioning of axes: three-dimensional alignment procedure for electron microscope tomography using three markers

R. Jonges; E. de Moor; P. N. Boon; J. van Marle; A. J. Dietrich; C. A. Grimbergen

doi:https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I

Three-point repositioning of axes: three-dimensional alignment procedure for electron microscope tomography using three markers

R. Jonges, E. de Moor, P. N. Boon, J. van Marle, A. J. Dietrich, C. A. Grimbergen

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Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

A description is given of a new procedure to align series of tilted graphs, made with an electron microscope, for computer tomographic purposes. The procedure uses the coordinates of three projected markers to calculate parameters needed for the reconstruction. To that end the procedure computes the direction of the tilt-axis, the translation and rotation parameters, the tilt-angle of every micrograph, and the spatial coordinates of the individual markers with their centre of gravity as origin of the coordinate system. A searching technique, based on cross-correlation, is described to locate accurately the micrographs markers

Original language	English
Pages (from-to)	516-526
Journal	MICROSCOPY RESEARCH AND TECHNIQUE
Volume	33
Issue number	6
DOIs	https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I
Publication status	Published - 1996

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https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I

Cite this

@article{c1ebc0dbd64d4926927134e38961e2c2,

title = "Three-point repositioning of axes: three-dimensional alignment procedure for electron microscope tomography using three markers",

abstract = "A description is given of a new procedure to align series of tilted graphs, made with an electron microscope, for computer tomographic purposes. The procedure uses the coordinates of three projected markers to calculate parameters needed for the reconstruction. To that end the procedure computes the direction of the tilt-axis, the translation and rotation parameters, the tilt-angle of every micrograph, and the spatial coordinates of the individual markers with their centre of gravity as origin of the coordinate system. A searching technique, based on cross-correlation, is described to locate accurately the micrographs markers",

author = "R. Jonges and {de Moor}, E. and Boon, {P. N.} and {van Marle}, J. and Dietrich, {A. J.} and Grimbergen, {C. A.}",

year = "1996",

doi = "https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I",

language = "English",

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pages = "516--526",

journal = "MICROSCOPY RESEARCH AND TECHNIQUE",

issn = "1059-910X",

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number = "6",

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T1 - Three-point repositioning of axes: three-dimensional alignment procedure for electron microscope tomography using three markers

AU - Jonges, R.

AU - de Moor, E.

AU - Boon, P. N.

AU - van Marle, J.

AU - Dietrich, A. J.

AU - Grimbergen, C. A.

PY - 1996

Y1 - 1996

N2 - A description is given of a new procedure to align series of tilted graphs, made with an electron microscope, for computer tomographic purposes. The procedure uses the coordinates of three projected markers to calculate parameters needed for the reconstruction. To that end the procedure computes the direction of the tilt-axis, the translation and rotation parameters, the tilt-angle of every micrograph, and the spatial coordinates of the individual markers with their centre of gravity as origin of the coordinate system. A searching technique, based on cross-correlation, is described to locate accurately the micrographs markers

AB - A description is given of a new procedure to align series of tilted graphs, made with an electron microscope, for computer tomographic purposes. The procedure uses the coordinates of three projected markers to calculate parameters needed for the reconstruction. To that end the procedure computes the direction of the tilt-axis, the translation and rotation parameters, the tilt-angle of every micrograph, and the spatial coordinates of the individual markers with their centre of gravity as origin of the coordinate system. A searching technique, based on cross-correlation, is described to locate accurately the micrographs markers

U2 - https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I

DO - https://doi.org/10.1002/(SICI)1097-0029(19960415)33:6<516::AID-JEMT7>3.0.CO;2-I

M3 - Article

C2 - 8800758

SN - 1059-910X

VL - 33

SP - 516

EP - 526

JO - MICROSCOPY RESEARCH AND TECHNIQUE

JF - MICROSCOPY RESEARCH AND TECHNIQUE

IS - 6

ER -