TY - JOUR
T1 - Tissue- and cell-specific distribution of creatine kinase B
T2 - A new and highly specific monoclonal antibody for use in immunohistochemistry
AU - Sistermans, Erik A.
AU - de Kok, Yvette J.M.
AU - Peters, Wilma
AU - Ginsel, Leo A.
AU - Jap, Paul H.K.
AU - Wieringa, Bé
PY - 1995/5
Y1 - 1995/5
N2 - A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as well as formaldehyde- or Bouin-fixed, paraffin-embedded sections of human, rabbit, and mouse tissues. Moreover, in the study of cell- and tissue-specific distribution patterns, parallel Western blot analysis and immunoelectron microscopy is possible using this antibody. Our analyses demonstrate that creatine kinase B expression is restricted to a specific subset of cell types in various tissues. In brain, the B-subunit was found only in neurocytes, but not in glia cells. High expression was also observed in inner segments of photoreceptor cells and the outer plexiform layer of the retina, in the parietal cells of the stomach and in gut enterocytes, gallbladder and epithelial cells of the urogenital system. The possible roles of the creatine kinase/phosphocreatine-ATP system in these tissues are discussed.
AB - A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as well as formaldehyde- or Bouin-fixed, paraffin-embedded sections of human, rabbit, and mouse tissues. Moreover, in the study of cell- and tissue-specific distribution patterns, parallel Western blot analysis and immunoelectron microscopy is possible using this antibody. Our analyses demonstrate that creatine kinase B expression is restricted to a specific subset of cell types in various tissues. In brain, the B-subunit was found only in neurocytes, but not in glia cells. High expression was also observed in inner segments of photoreceptor cells and the outer plexiform layer of the retina, in the parietal cells of the stomach and in gut enterocytes, gallbladder and epithelial cells of the urogenital system. The possible roles of the creatine kinase/phosphocreatine-ATP system in these tissues are discussed.
KW - B-subunit
KW - Creatine kinase
KW - Immuno-electron microscopy
KW - Immunohistochemistry
KW - Monoclonal antibody
KW - Mouse (C57BL/6)
KW - Rabbit (New Zealand White)
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=0028905460&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/BF00307817
DO - https://doi.org/10.1007/BF00307817
M3 - Article
C2 - 7781040
SN - 0302-766X
VL - 280
SP - 435
EP - 446
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -