Toll-like receptor 4 is not involved in host defense against respiratory tract infection with Sendai virus

Koenraad F. van der Sluijs; Leontine van Elden; Monique Nijhuis; Rob Schuurman; Sandrine Florquin; Henk M. Jansen; René Lutter; Tom van der Poll

doi:https://doi.org/10.1016/S0165-2478(03)00138-X

Toll-like receptor 4 is not involved in host defense against respiratory tract infection with Sendai virus

Koenraad F. van der Sluijs, Leontine van Elden, Monique Nijhuis, Rob Schuurman, Sandrine Florquin, Henk M. Jansen, René Lutter, Tom van der Poll

Research output: Contribution to journal › Article › Academic › peer-review

35 Citations (Scopus)

Abstract

Toll-like receptors (TLR) induce innate immune responses upon stimulation by a wide variety of pathogens. TLR4 has been implicated in innate immunity against respiratory syncytial virus (RSV) by an interaction with the viral envelope fusion (F) protein. Sendai virus (mouse parainfluenza type 1) shares many features with RSV, including a structurally and functionally similar F protein. To determine the role of TLR4 in host defense against Sendai virus respiratory tract infection, TLR4 mutant and wildtype mice were intranasally infected with Sendai virus. Sendai infection resulted in an increase in viral RNA copies in lung homogenates peaking on day 4. Pulmonary viral loads, histopathology, cytokine levels and leukocyte influx were similar in TLR4 mutant and wildtype mice. In spite of the structural similarities shared by the F proteins of Sendai virus and RSV, TLR4 is not involved in host defense against respiratory tract infection with Sendai virus. (C) 2003 Published by Elsevier B.V

Original language	English
Pages (from-to)	201-206
Journal	Immunology letters
Volume	89
Issue number	2-3
DOIs	https://doi.org/10.1016/S0165-2478(03)00138-X
Publication status	Published - 2003

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https://doi.org/10.1016/S0165-2478(03)00138-X

Cite this

@article{dc34bb7c415c46fea695a26d90050f71,

title = "Toll-like receptor 4 is not involved in host defense against respiratory tract infection with Sendai virus",

abstract = "Toll-like receptors (TLR) induce innate immune responses upon stimulation by a wide variety of pathogens. TLR4 has been implicated in innate immunity against respiratory syncytial virus (RSV) by an interaction with the viral envelope fusion (F) protein. Sendai virus (mouse parainfluenza type 1) shares many features with RSV, including a structurally and functionally similar F protein. To determine the role of TLR4 in host defense against Sendai virus respiratory tract infection, TLR4 mutant and wildtype mice were intranasally infected with Sendai virus. Sendai infection resulted in an increase in viral RNA copies in lung homogenates peaking on day 4. Pulmonary viral loads, histopathology, cytokine levels and leukocyte influx were similar in TLR4 mutant and wildtype mice. In spite of the structural similarities shared by the F proteins of Sendai virus and RSV, TLR4 is not involved in host defense against respiratory tract infection with Sendai virus. (C) 2003 Published by Elsevier B.V",

author = "{van der Sluijs}, {Koenraad F.} and {van Elden}, Leontine and Monique Nijhuis and Rob Schuurman and Sandrine Florquin and Jansen, {Henk M.} and Ren{\'e} Lutter and {van der Poll}, Tom",

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T1 - Toll-like receptor 4 is not involved in host defense against respiratory tract infection with Sendai virus

AU - van der Sluijs, Koenraad F.

AU - van Elden, Leontine

AU - Nijhuis, Monique

AU - Schuurman, Rob

AU - Florquin, Sandrine

AU - Jansen, Henk M.

AU - Lutter, René

AU - van der Poll, Tom

PY - 2003

Y1 - 2003

N2 - Toll-like receptors (TLR) induce innate immune responses upon stimulation by a wide variety of pathogens. TLR4 has been implicated in innate immunity against respiratory syncytial virus (RSV) by an interaction with the viral envelope fusion (F) protein. Sendai virus (mouse parainfluenza type 1) shares many features with RSV, including a structurally and functionally similar F protein. To determine the role of TLR4 in host defense against Sendai virus respiratory tract infection, TLR4 mutant and wildtype mice were intranasally infected with Sendai virus. Sendai infection resulted in an increase in viral RNA copies in lung homogenates peaking on day 4. Pulmonary viral loads, histopathology, cytokine levels and leukocyte influx were similar in TLR4 mutant and wildtype mice. In spite of the structural similarities shared by the F proteins of Sendai virus and RSV, TLR4 is not involved in host defense against respiratory tract infection with Sendai virus. (C) 2003 Published by Elsevier B.V

AB - Toll-like receptors (TLR) induce innate immune responses upon stimulation by a wide variety of pathogens. TLR4 has been implicated in innate immunity against respiratory syncytial virus (RSV) by an interaction with the viral envelope fusion (F) protein. Sendai virus (mouse parainfluenza type 1) shares many features with RSV, including a structurally and functionally similar F protein. To determine the role of TLR4 in host defense against Sendai virus respiratory tract infection, TLR4 mutant and wildtype mice were intranasally infected with Sendai virus. Sendai infection resulted in an increase in viral RNA copies in lung homogenates peaking on day 4. Pulmonary viral loads, histopathology, cytokine levels and leukocyte influx were similar in TLR4 mutant and wildtype mice. In spite of the structural similarities shared by the F proteins of Sendai virus and RSV, TLR4 is not involved in host defense against respiratory tract infection with Sendai virus. (C) 2003 Published by Elsevier B.V

U2 - https://doi.org/10.1016/S0165-2478(03)00138-X

DO - https://doi.org/10.1016/S0165-2478(03)00138-X

M3 - Article

C2 - 14556979

SN - 0165-2478

VL - 89

SP - 201

EP - 206

JO - Immunology letters

JF - Immunology letters

IS - 2-3

ER -