TY - JOUR
T1 - Toward a Durable Anti-HIV Gene Therapy Based on RNA Interference
AU - Berkhout, Ben
PY - 2009
Y1 - 2009
N2 - Basic research in the field of molecular biology led to the discovery of the mechanism of RNA interference (RNAi) in Caenorhabditis elegans in 1998. RNAi is now widely appreciated as an important gene control mechanism in mammals, and several RNAi-based gene-silencing applications have already been used in clinical trials. In this review I will discuss RNAi approaches to inhibit the pathogenic human immunodeficiency virus type I (HIV-1), which establishes a chronic infection that would most likely require a durable gene therapy approach. Viruses, such as HIV-1, are particularly difficult targets for RNAi attack because they mutate frequently, which allows viral escape by mutation of the RNAi target sequence. Combinatorial RNAi strategies are required to prevent viral escape
AB - Basic research in the field of molecular biology led to the discovery of the mechanism of RNA interference (RNAi) in Caenorhabditis elegans in 1998. RNAi is now widely appreciated as an important gene control mechanism in mammals, and several RNAi-based gene-silencing applications have already been used in clinical trials. In this review I will discuss RNAi approaches to inhibit the pathogenic human immunodeficiency virus type I (HIV-1), which establishes a chronic infection that would most likely require a durable gene therapy approach. Viruses, such as HIV-1, are particularly difficult targets for RNAi attack because they mutate frequently, which allows viral escape by mutation of the RNAi target sequence. Combinatorial RNAi strategies are required to prevent viral escape
U2 - https://doi.org/10.1111/j.1749-6632.2009.04972.x
DO - https://doi.org/10.1111/j.1749-6632.2009.04972.x
M3 - Article
C2 - 19796072
SN - 0077-8923
VL - 1175
SP - 3
EP - 14
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -