TY - JOUR
T1 - Towards Full Thickness Small Intestinal Models
T2 - Incorporation of Stromal Cells
AU - Asal, Melis
AU - Rep, Mila
AU - Bontkes, Hetty J.
AU - van Vliet, Sandra J.
AU - Mebius, Reina E.
AU - Gibbs, Susan
N1 - Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation program under Grant Agreement No 847551. Publisher Copyright: © 2023, The Author(s).
PY - 2023
Y1 - 2023
N2 - Introduction: Since small intestine is one of the major barriers of the human body, there is a need to develop reliable in vitro human small intestinal models. These models should incorporate both the epithelial and lamina propria compartments and have similar barrier properties compared to that of the human tissue. These properties are essential for various applications, such as studying cell–cell interaction, intestinal diseases and testing permeability and metabolism of drugs and other compounds. The small intestinal lamina propria contains multiple stromal cell populations with several important functions, such as secretion of extracellular matrix proteins and soluble mediators. In addition, stromal cells influence the intestinal epithelial barrier, support the intestinal stem cell niche and interact with immune cells. Methods: In this review, we provide an extensive overview on the different types of lamina propria stromal cells found in small intestine and describe a combination of molecular markers that can be used to distinguish each different stromal cell type. We focus on studies that incorporated stromal cells into human representative small intestine models cultured on transwells. Results and Conclusion: These models display enhanced epithelial morphology, increased cell proliferation and human-like barrier properties, such as low transepithelial electrical resistance (TEER) and intermediate permeability, thus better mimicking the native human small intestine than models only consisting of an epithelium which generally show high TEER and low permeability.
AB - Introduction: Since small intestine is one of the major barriers of the human body, there is a need to develop reliable in vitro human small intestinal models. These models should incorporate both the epithelial and lamina propria compartments and have similar barrier properties compared to that of the human tissue. These properties are essential for various applications, such as studying cell–cell interaction, intestinal diseases and testing permeability and metabolism of drugs and other compounds. The small intestinal lamina propria contains multiple stromal cell populations with several important functions, such as secretion of extracellular matrix proteins and soluble mediators. In addition, stromal cells influence the intestinal epithelial barrier, support the intestinal stem cell niche and interact with immune cells. Methods: In this review, we provide an extensive overview on the different types of lamina propria stromal cells found in small intestine and describe a combination of molecular markers that can be used to distinguish each different stromal cell type. We focus on studies that incorporated stromal cells into human representative small intestine models cultured on transwells. Results and Conclusion: These models display enhanced epithelial morphology, increased cell proliferation and human-like barrier properties, such as low transepithelial electrical resistance (TEER) and intermediate permeability, thus better mimicking the native human small intestine than models only consisting of an epithelium which generally show high TEER and low permeability.
KW - Human small intestine
KW - In vitro model
KW - Lamina propria
KW - Stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85180261802&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s13770-023-00600-6
DO - https://doi.org/10.1007/s13770-023-00600-6
M3 - Review article
C2 - 38113015
SN - 1738-2696
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
ER -