TY - JOUR
T1 - Transfusion of female blood in a rat model is associated with red blood cells entrapment in organs
AU - Alshalani, Abdulrahman
AU - de Wissel, Marit B
AU - Tuip-de Boer, Anita M
AU - Roelofs, Joris J T H
AU - van Bruggen, Robin
AU - Acker, Jason P
AU - Juffermans, Nicole P
N1 - Funding Information: We would like to acknowledge staff of the animal facility at Amsterdam University Medical Center for their help in housing animals used in this experiment. Abdulrahman Alshalani is supported by the Deanship of Scientific Research, King Saud University through the Vice Deanship of Scientific Research Chairs; Research Chair of Medical and Molecular Genetics. Publisher Copyright: © 2023 Alshalani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2023/11
Y1 - 2023/11
N2 - Transfusion of red blood cells (RBCs) has been associated with adverse outcomes. Mechanisms may be related to donor sex and biological age of RBC. This study hypothesized that receipt of female blood is associated with decreased post-transfusion recovery (PTR) and a concomitant increased organ entrapment in rats, related to young age of donor RBCs. Donor rats underwent bloodletting to stimulate production of new, young RBCs, followed by Percoll fractionation for further enrichment of young RBCs based on their low density. Control donors did not undergo these procedures. Male rats received either a (biotinylated) standard RBC product or a product enriched for young RBCs, derived from either male or female donors. Controls received saline. Organs and blood samples were harvested after 24 hours. This study found no difference in PTR between groups, although only the group receiving young RBCs from females failed to reach a PTR of 75%. Receipt of both standard RBCs and young RBCs from females was associated with increased entrapment of donor RBCs in the lung, liver, and spleen compared to receiving blood from male donors. Soluble ICAM-1 and markers of hemolysis were higher in recipients of female blood compared to control. In conclusion, transfusing RBCs from female donors, but not from male donors, is associated with trapping of donor RBCs in organs, accompanied by endothelial activation and hemolysis.
AB - Transfusion of red blood cells (RBCs) has been associated with adverse outcomes. Mechanisms may be related to donor sex and biological age of RBC. This study hypothesized that receipt of female blood is associated with decreased post-transfusion recovery (PTR) and a concomitant increased organ entrapment in rats, related to young age of donor RBCs. Donor rats underwent bloodletting to stimulate production of new, young RBCs, followed by Percoll fractionation for further enrichment of young RBCs based on their low density. Control donors did not undergo these procedures. Male rats received either a (biotinylated) standard RBC product or a product enriched for young RBCs, derived from either male or female donors. Controls received saline. Organs and blood samples were harvested after 24 hours. This study found no difference in PTR between groups, although only the group receiving young RBCs from females failed to reach a PTR of 75%. Receipt of both standard RBCs and young RBCs from females was associated with increased entrapment of donor RBCs in the lung, liver, and spleen compared to receiving blood from male donors. Soluble ICAM-1 and markers of hemolysis were higher in recipients of female blood compared to control. In conclusion, transfusing RBCs from female donors, but not from male donors, is associated with trapping of donor RBCs in organs, accompanied by endothelial activation and hemolysis.
KW - Animals
KW - Blood Donors
KW - Blood Preservation/methods
KW - Blood Transfusion
KW - Erythrocyte Transfusion/adverse effects
KW - Erythrocytes
KW - Female
KW - Hemolysis
KW - Male
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=85177851961&partnerID=8YFLogxK
U2 - https://doi.org/10.1371/journal.pone.0288308
DO - https://doi.org/10.1371/journal.pone.0288308
M3 - Article
C2 - 37992035
SN - 1932-6203
VL - 18
SP - e0288308
JO - PLOS ONE
JF - PLOS ONE
IS - 11 November
M1 - e0288308
ER -