Two Photon Confocal Microscopy in wound healing

Fernando A. Navarro; Peter T. C. So; Antoine Driessen; Nina Kropf; Christine S. Park; Juan C. Huertas; Hoon B. Lee; Dennis P. Orgill

doi:https://doi.org/10.1117/12.424579

Two Photon Confocal Microscopy in wound healing

Fernando A. Navarro, Peter T. C. So, Antoine Driessen, Nina Kropf, Christine S. Park, Juan C. Huertas, Hoon B. Lee, Dennis P. Orgill

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Advances in histopathology and immunohistochemestry have allowed for precise microanatomic detail of tissues. Two Photon Confocal Microscopy (TPCM) is a new technology useful in non-destructive analysis of tissue. Laser light excites the natural florophores, NAD(P)H and NADP+ and the scattering patterns of the emitted light are analyzed to reconstruct microanatomic features. Guinea pig skin was studied using TPCM and skin preparation methods including chemical depilation and tape stripping. Results of TPCM were compared with conventional hematoxylin and eosin microscopy. Two-dimensional images were rendered from the three dimensional reconstructions. Images of deeper layers including basal cells and the dermo-epidermal junction improved after removing the stratum corneum with chemical depilation or tape stripping. TCPM allows good resolution of corneocytes, basal cells and collagen fibers and shows promise as a non-destructive method to study wound healing.

Original language	English
Pages (from-to)	27-40
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4262
DOIs	https://doi.org/10.1117/12.424579
Publication status	Published - 2001
Externally published	Yes

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title = "Two Photon Confocal Microscopy in wound healing",

abstract = "Advances in histopathology and immunohistochemestry have allowed for precise microanatomic detail of tissues. Two Photon Confocal Microscopy (TPCM) is a new technology useful in non-destructive analysis of tissue. Laser light excites the natural florophores, NAD(P)H and NADP+ and the scattering patterns of the emitted light are analyzed to reconstruct microanatomic features. Guinea pig skin was studied using TPCM and skin preparation methods including chemical depilation and tape stripping. Results of TPCM were compared with conventional hematoxylin and eosin microscopy. Two-dimensional images were rendered from the three dimensional reconstructions. Images of deeper layers including basal cells and the dermo-epidermal junction improved after removing the stratum corneum with chemical depilation or tape stripping. TCPM allows good resolution of corneocytes, basal cells and collagen fibers and shows promise as a non-destructive method to study wound healing.",

author = "Navarro, {Fernando A.} and So, {Peter T. C.} and Antoine Driessen and Nina Kropf and Park, {Christine S.} and Huertas, {Juan C.} and Lee, {Hoon B.} and Orgill, {Dennis P.}",

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AU - Navarro, Fernando A.

AU - So, Peter T. C.

AU - Driessen, Antoine

AU - Kropf, Nina

AU - Park, Christine S.

AU - Huertas, Juan C.

AU - Lee, Hoon B.

AU - Orgill, Dennis P.

PY - 2001

Y1 - 2001

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AB - Advances in histopathology and immunohistochemestry have allowed for precise microanatomic detail of tissues. Two Photon Confocal Microscopy (TPCM) is a new technology useful in non-destructive analysis of tissue. Laser light excites the natural florophores, NAD(P)H and NADP+ and the scattering patterns of the emitted light are analyzed to reconstruct microanatomic features. Guinea pig skin was studied using TPCM and skin preparation methods including chemical depilation and tape stripping. Results of TPCM were compared with conventional hematoxylin and eosin microscopy. Two-dimensional images were rendered from the three dimensional reconstructions. Images of deeper layers including basal cells and the dermo-epidermal junction improved after removing the stratum corneum with chemical depilation or tape stripping. TCPM allows good resolution of corneocytes, basal cells and collagen fibers and shows promise as a non-destructive method to study wound healing.

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JF - Proceedings of SPIE - The International Society for Optical Engineering

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Two Photon Confocal Microscopy in wound healing

Abstract

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