TY - JOUR
T1 - Urinary exosomal proteins as (pan-)cancer biomarkers: insights from the proteome
AU - Erozenci, Leyla Ayse
AU - Böttger, Franziska
AU - Bijnsdorp, Irene V.
AU - Jimenez, Connie R.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Exosomes are extracellular vesicles (EVs) released from cells under both physiological and pathological conditions, and may, thus, be present in biofluids. Urine is one of the most accessible biofluids implemented in clinical diagnostics. Recent mass spectrometry (MS)-based proteomic analyses have enabled high-throughput, deep proteome profiling of urinary EVs for the discovery, quantification and characterization of cancer-specific exosome biomarkers. The protein cargo of urine exosomes is emerging as an attractive source for biomarkers, not only for urological cancers, such as prostate, bladder and kidney cancer, but potentially also for nonurological cancers, including gastric, lung, oesophageal and colorectal cancer. More recently, exosome proteomics dissected protein cargo in the lumen and at the surface of EVs, and unexpectedly indicated that RNA- and DNA-binding proteins might also be present on vesicular surfaces. Here, we analyse MS-based proteomic data on urinary exosomes from cancer patients, and discuss the potential of urinary exosome-derived biomarkers in cancer.
AB - Exosomes are extracellular vesicles (EVs) released from cells under both physiological and pathological conditions, and may, thus, be present in biofluids. Urine is one of the most accessible biofluids implemented in clinical diagnostics. Recent mass spectrometry (MS)-based proteomic analyses have enabled high-throughput, deep proteome profiling of urinary EVs for the discovery, quantification and characterization of cancer-specific exosome biomarkers. The protein cargo of urine exosomes is emerging as an attractive source for biomarkers, not only for urological cancers, such as prostate, bladder and kidney cancer, but potentially also for nonurological cancers, including gastric, lung, oesophageal and colorectal cancer. More recently, exosome proteomics dissected protein cargo in the lumen and at the surface of EVs, and unexpectedly indicated that RNA- and DNA-binding proteins might also be present on vesicular surfaces. Here, we analyse MS-based proteomic data on urinary exosomes from cancer patients, and discuss the potential of urinary exosome-derived biomarkers in cancer.
KW - cancer
KW - exosomes
KW - extracellular vesicles
KW - mass spectrometry
KW - proteomics
KW - urine
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069950588&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31198995
U2 - https://doi.org/10.1002/1873-3468.13487
DO - https://doi.org/10.1002/1873-3468.13487
M3 - Article
C2 - 31198995
SN - 0014-5793
VL - 593
SP - 1580
EP - 1597
JO - FEBS letters
JF - FEBS letters
IS - 13
ER -