TY - JOUR
T1 - Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
AU - Erdbrügger, Uta
AU - Blijdorp, Charles J.
AU - Bijnsdorp, Irene V.
AU - Borràs, Francesc E.
AU - Burger, Dylan
AU - Bussolati, Benedetta
AU - Byrd, James Brian
AU - Clayton, Aled
AU - Dear, James W.
AU - Falcón-Pérez, Juan M.
AU - Grange, Cristina
AU - Hill, Andrew F.
AU - Holthöfer, Harry
AU - Hoorn, Ewout J.
AU - Jenster, Guido
AU - Jimenez, Connie R.
AU - Junker, Kerstin
AU - Klein, John
AU - Knepper, Mark A.
AU - Koritzinsky, Erik H.
AU - Luther, James M.
AU - Lenassi, Metka
AU - Leivo, Janne
AU - Mertens, Inge
AU - Musante, Luca
AU - Oeyen, Eline
AU - Puhka, Maija
AU - van Royen, Martin E.
AU - Sánchez, Catherine
AU - Soekmadji, Carolina
AU - Thongboonkerd, Visith
AU - van Steijn, Volkert
AU - Verhaegh, Gerald
AU - Webber, Jason P.
AU - Witwer, Kenneth
AU - Yuen, Peter S. T.
AU - Zheng, Lei
AU - Llorente, Alicia
AU - Martens-Uzunova, Elena S.
N1 - Funding Information: ESM‐U, CG, GV, GJ, IVB, MvR, and VvS, are members of the “IMMPROVE” consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015‐8022). Funding Information: We thank Karina Barreiro for the transmission electron microscopy images courtesy of Beat DKD project under IMI2, Dr. Kelly Dryden, PhD at the UVA core facility for the cryo-EM images and Dr Benedetta Bussolati, MD for the Super resolution dSTORM images of EVs. Other figures in this manuscript have been created with BioRender.com ESM-U, CG, GV, GJ, IVB, MvR, and VvS, are members of the ?IMMPROVE? consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015-8022). AL is supported by Norges Forskningsr?d, Kreftforeningen and Helse S?r-?st RHF (NO). UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23-HL-126101. CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05. Funding Information: UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23‐HL‐126101. Funding Information: AL is supported by Norges Forskningsråd, Kreftforeningen and Helse Sør‐Øst RHF (NO). Funding Information: CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05. Publisher Copyright: © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
AB - Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
KW - biobank
KW - biomarkers
KW - bladder
KW - extracellular vesicles
KW - kidney
KW - liquid biopsy
KW - prostate
KW - rigor and standardization
KW - urine
UR - http://www.scopus.com/inward/record.url?scp=85106448800&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/jev2.12093
DO - https://doi.org/10.1002/jev2.12093
M3 - Article
C2 - 34035881
SN - 2001-3078
VL - 10
JO - Journal of extracellular vesicles
JF - Journal of extracellular vesicles
IS - 7
M1 - e12093
ER -