Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures

Selami Baglamis; Vivek M. Sheraton; Debora Meijer; Haibin Qian; Ron A. Hoebe; Kristiaan J. Lenos; Max A. Betjes; Max A. Betjes; Sander Tans; Jeroen van Zon; Louis Vermeulen; Przemek M. Krawczyk

doi:https://doi.org/10.1038/s41598-023-42849-w

Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures

Selami Baglamis, Vivek M. Sheraton, Debora Meijer, Haibin Qian, Ron A. Hoebe, Kristiaan J. Lenos, Max A. Betjes, Max A. Betjes, Sander Tans, Jeroen van Zon, Louis Vermeulen, Przemek M. Krawczyk

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in monoculture conditions, as well as in co-cultures with stromal fibroblasts.

Original language	English
Article number	18832
Pages (from-to)	18832
Journal	Scientific reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-42849-w
Publication status	Published - 1 Dec 2023

Keywords

Cell Culture Techniques
Cell Line
Clone Cells
Coculture Techniques
Humans
Neoplasms

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Baglamis, S., Sheraton, V. M., Meijer, D., Qian, H., Hoebe, R. A., Lenos, K. J., Betjes, M. A., Betjes, M. A., Tans, S., van Zon, J., Vermeulen, L., & Krawczyk, P. M. (2023). Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures. Scientific reports, 13(1), 18832. Article 18832. https://doi.org/10.1038/s41598-023-42849-w

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title = "Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures",

abstract = "Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in monoculture conditions, as well as in co-cultures with stromal fibroblasts.",

keywords = "Cell Culture Techniques, Cell Line, Clone Cells, Coculture Techniques, Humans, Neoplasms",

author = "Selami Baglamis and Sheraton, {Vivek M.} and Debora Meijer and Haibin Qian and Hoebe, {Ron A.} and Lenos, {Kristiaan J.} and Betjes, {Max A.} and Betjes, {Max A.} and Sander Tans and {van Zon}, Jeroen and Louis Vermeulen and Krawczyk, {Przemek M.}",

note = "Funding Information: S.B. is grateful for the scholarship from the Republic of Turkey, Ministry of National Education. This work is supported by Oncode Institute, The New York Stem Cell Foundation, grants from the European Research Council (ERC-CoG 101045612—NIMICRY) and ZonMw (Vici 09-15018-21-10029) to L.V. L.V. is a New York Stem Cell Foundation—Robertson Investigator. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

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doi = "https://doi.org/10.1038/s41598-023-42849-w",

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AU - Baglamis, Selami

AU - Sheraton, Vivek M.

AU - Meijer, Debora

AU - Qian, Haibin

AU - Hoebe, Ron A.

AU - Lenos, Kristiaan J.

AU - Betjes, Max A.

AU - Tans, Sander

AU - van Zon, Jeroen

AU - Vermeulen, Louis

AU - Krawczyk, Przemek M.

N1 - Funding Information: S.B. is grateful for the scholarship from the Republic of Turkey, Ministry of National Education. This work is supported by Oncode Institute, The New York Stem Cell Foundation, grants from the European Research Council (ERC-CoG 101045612—NIMICRY) and ZonMw (Vici 09-15018-21-10029) to L.V. L.V. is a New York Stem Cell Foundation—Robertson Investigator. Publisher Copyright: © 2023, The Author(s).

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in monoculture conditions, as well as in co-cultures with stromal fibroblasts.

AB - Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional cell cultures. The method allows for the simultaneous visualization and analysis of multiple clones in individual multi-clonal colonies, providing a powerful tool for studying clonal dynamics and identifying clonal populations with distinct characteristics. Results of our experiments validate the utility of the method in studying clonal dynamics in vitro, and reveal differences in key aspects of clonal behavior of different cancer cell lines in monoculture conditions, as well as in co-cultures with stromal fibroblasts.

KW - Cell Culture Techniques

KW - Cell Line

KW - Clone Cells

KW - Coculture Techniques

KW - Humans

KW - Neoplasms

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SN - 2045-2322

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SP - 18832

JO - Scientific reports

JF - Scientific reports

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ER -

Using picoliter droplet deposition to track clonal competition in adherent and organoid cancer cell cultures

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Keywords

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