TY - JOUR
T1 - Viral dsRNA-activated human dendritic cells produce IL-27, which selectively promotes cytotoxicity in naive CD8(+) T cells
AU - de Groot, Rosa
AU - van Beelen, Astrid J.
AU - Bakdash, Ghaith
AU - Taanman-Kueter, Esther W. M.
AU - de Jong, Esther C.
AU - Kapsenberg, Martien L.
PY - 2012
Y1 - 2012
N2 - Viral recognition programs DCs to express Signal 3 molecules that promote the differentiation of effector CD8(+) T cells. Besides IL-12, another DC-derived IL-12 family member, IL-27, has been reported to contribute herein, but its specific role is not well understood. Here, we show that whereas IL-12 potently induces inflammatory cytokines (i.e., IFN-gamma and TNF-alpha, but not IL-2), IL-27 excels in inducing proliferation and a cytotoxic profile (GrB, cytotoxicity of target cells) in human naive CD8(+) T cells. Compared with bacterial cell-wall peptidoglycan, viral dsRNA-mimic poly (I:C) is superior in priming human BDCA1(+) peripheral blood DCs to produce IL-12 and IL-27, which promote inflammatory cytokines and a cytotoxic profile in differentiating CD8(+) T cells, respectively. These data support the concept that viral dsRNA-activated human DCs produce IL-27 to act as a specialized procytotoxic, antiviral cytokine in the development of effector CD8(+) T cells. J. Leukoc. Biol. 92: 605-610; 2012
AB - Viral recognition programs DCs to express Signal 3 molecules that promote the differentiation of effector CD8(+) T cells. Besides IL-12, another DC-derived IL-12 family member, IL-27, has been reported to contribute herein, but its specific role is not well understood. Here, we show that whereas IL-12 potently induces inflammatory cytokines (i.e., IFN-gamma and TNF-alpha, but not IL-2), IL-27 excels in inducing proliferation and a cytotoxic profile (GrB, cytotoxicity of target cells) in human naive CD8(+) T cells. Compared with bacterial cell-wall peptidoglycan, viral dsRNA-mimic poly (I:C) is superior in priming human BDCA1(+) peripheral blood DCs to produce IL-12 and IL-27, which promote inflammatory cytokines and a cytotoxic profile in differentiating CD8(+) T cells, respectively. These data support the concept that viral dsRNA-activated human DCs produce IL-27 to act as a specialized procytotoxic, antiviral cytokine in the development of effector CD8(+) T cells. J. Leukoc. Biol. 92: 605-610; 2012
U2 - https://doi.org/10.1189/jlb.0112045
DO - https://doi.org/10.1189/jlb.0112045
M3 - Article
C2 - 22701040
SN - 0741-5400
VL - 92
SP - 605
EP - 610
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
IS - 3
ER -