TY - JOUR
T1 - Wilms' tumor gene 1 (WT1) in endometrial carcinoma
AU - Coosemans, An
AU - Moerman, Philippe
AU - Verbist, Godelieve
AU - Maes, Wim
AU - Neven, Patrick
AU - Vergote, Ignace
AU - van Gool, Stefaan W.
AU - Amant, Frédéric
PY - 2008
Y1 - 2008
N2 - Objective. Wilms' tumor gene (WT1), located on chromosome 11, encodes a transcription factor that contributes to the carcinogenesis of uterine sarcomas. To expand the knowledge on the biological role of WT1 in other uterine cancers, we focused on its detection in endometrial carcinoma. Methods. In total, 36 paraffin-embedded tumors were available for WT1 immunohistochemical (IHC) analysis including endometrial endometrioid carcinoma (n=24), serous carcinoma (n=9) and clear cell carcinoma (n=3). Three slides from different sites of the tumor were analysed. Of these tumors, 32 snap frozen tissue samples were available for RT-PCR (endometrioid carcinoma (23), serous carcinoma (7) and clear cell carcinoma (2)). To compare, WT1 expression was also evaluated by IHC in benign endometrium (12) and benign endometrial polyps (5). Results. WT1 positivity was noticed in tumor cells and endothelial cells, lining the intratumoral blood vessels. Overall, 72% (26/36) of tumors stained positive for WT1. RT-PCR results showed WT1 positivity in 75% (24/32) of samples. Comparing the staining patterns of the 3 different bioptic sites, tumor heterogeneity was demonstrated in the majority (72%) of samples. Conclusion. Although WT1 is expressed in a majority of endometrial carcinomas, a heterogeneous staining patient is observed. This information is important for WT1-directed immunotherapy. (C) 2008 Published by Elsevier Inc
AB - Objective. Wilms' tumor gene (WT1), located on chromosome 11, encodes a transcription factor that contributes to the carcinogenesis of uterine sarcomas. To expand the knowledge on the biological role of WT1 in other uterine cancers, we focused on its detection in endometrial carcinoma. Methods. In total, 36 paraffin-embedded tumors were available for WT1 immunohistochemical (IHC) analysis including endometrial endometrioid carcinoma (n=24), serous carcinoma (n=9) and clear cell carcinoma (n=3). Three slides from different sites of the tumor were analysed. Of these tumors, 32 snap frozen tissue samples were available for RT-PCR (endometrioid carcinoma (23), serous carcinoma (7) and clear cell carcinoma (2)). To compare, WT1 expression was also evaluated by IHC in benign endometrium (12) and benign endometrial polyps (5). Results. WT1 positivity was noticed in tumor cells and endothelial cells, lining the intratumoral blood vessels. Overall, 72% (26/36) of tumors stained positive for WT1. RT-PCR results showed WT1 positivity in 75% (24/32) of samples. Comparing the staining patterns of the 3 different bioptic sites, tumor heterogeneity was demonstrated in the majority (72%) of samples. Conclusion. Although WT1 is expressed in a majority of endometrial carcinomas, a heterogeneous staining patient is observed. This information is important for WT1-directed immunotherapy. (C) 2008 Published by Elsevier Inc
U2 - https://doi.org/10.1016/j.ygyno.2008.08.032
DO - https://doi.org/10.1016/j.ygyno.2008.08.032
M3 - Article
C2 - 18929401
SN - 0090-8258
VL - 111
SP - 502
EP - 508
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 3
ER -