Y chromosome TSPY copy numbers and semen quality

Bita Nickkholgh; Michiel J. Noordam; Suzanne E. Hovingh; Ans M. M. van Pelt; Fulco van der Veen; Sjoerd Repping

doi:https://doi.org/10.1016/j.fertnstert.2009.09.051

Y chromosome TSPY copy numbers and semen quality

Bita Nickkholgh, Michiel J. Noordam, Suzanne E. Hovingh, Ans M. M. van Pelt, Fulco van der Veen, Sjoerd Repping

Research output: Contribution to journal › Article › Academic › peer-review

31 Citations (Scopus)

Abstract

Objective: To determine whether variation in testis-specific protein Y-encoded (TSPY) gene copy number affects semen quality. Design: Nested case-control study. Setting: University hospital. Patient(s): From a consecutive cohort of 1,016 male partners of subfertile couples, unselected for sperm counts, we selected as cases 100 men with the lowest total number of progressively motile sperm (TMC) and as controls, 100 men with the highest total number of progressively motile sperm. Intervention(s): Quantitative real-time polymerase chain reaction (PCR) and Southern blot to determine TSPY copy number. Main Outcome Measure(s): TSPY copy number. Result(s): The quantitative PCR method showed excellent agreement with the Southern blot analysis. Cases had a median TSPY copy number of 35 (range 20-73), whereas controls had a median TSPY copy number of 34 (range 26-76). This difference was not statistically significant. Conclusion(s): We found no association between TSPY copy numbers and severe spermatogenic failure. The observed variation in TSPY copy number therefore appears to have no functional consequences for semen quality. (Fertil Steril (R) 2010;94:1744-7. (C)2010 by American Society for Reproductive Medicine.)

Original language	English
Pages (from-to)	1744-1747
Journal	Fertility and Sterility
Volume	94
Issue number	5
DOIs	https://doi.org/10.1016/j.fertnstert.2009.09.051
Publication status	Published - 2010

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https://doi.org/10.1016/j.fertnstert.2009.09.051

Cite this

@article{aa2c6d1392d34922b14bce920fbea4bc,

title = "Y chromosome TSPY copy numbers and semen quality",

abstract = "Objective: To determine whether variation in testis-specific protein Y-encoded (TSPY) gene copy number affects semen quality. Design: Nested case-control study. Setting: University hospital. Patient(s): From a consecutive cohort of 1,016 male partners of subfertile couples, unselected for sperm counts, we selected as cases 100 men with the lowest total number of progressively motile sperm (TMC) and as controls, 100 men with the highest total number of progressively motile sperm. Intervention(s): Quantitative real-time polymerase chain reaction (PCR) and Southern blot to determine TSPY copy number. Main Outcome Measure(s): TSPY copy number. Result(s): The quantitative PCR method showed excellent agreement with the Southern blot analysis. Cases had a median TSPY copy number of 35 (range 20-73), whereas controls had a median TSPY copy number of 34 (range 26-76). This difference was not statistically significant. Conclusion(s): We found no association between TSPY copy numbers and severe spermatogenic failure. The observed variation in TSPY copy number therefore appears to have no functional consequences for semen quality. (Fertil Steril (R) 2010;94:1744-7. (C)2010 by American Society for Reproductive Medicine.)",

author = "Bita Nickkholgh and Noordam, {Michiel J.} and Hovingh, {Suzanne E.} and {van Pelt}, {Ans M. M.} and {van der Veen}, Fulco and Sjoerd Repping",

year = "2010",

doi = "https://doi.org/10.1016/j.fertnstert.2009.09.051",

language = "English",

volume = "94",

pages = "1744--1747",

journal = "Fertility and Sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Y chromosome TSPY copy numbers and semen quality

AU - Nickkholgh, Bita

AU - Noordam, Michiel J.

AU - Hovingh, Suzanne E.

AU - van Pelt, Ans M. M.

AU - van der Veen, Fulco

AU - Repping, Sjoerd

PY - 2010

Y1 - 2010

N2 - Objective: To determine whether variation in testis-specific protein Y-encoded (TSPY) gene copy number affects semen quality. Design: Nested case-control study. Setting: University hospital. Patient(s): From a consecutive cohort of 1,016 male partners of subfertile couples, unselected for sperm counts, we selected as cases 100 men with the lowest total number of progressively motile sperm (TMC) and as controls, 100 men with the highest total number of progressively motile sperm. Intervention(s): Quantitative real-time polymerase chain reaction (PCR) and Southern blot to determine TSPY copy number. Main Outcome Measure(s): TSPY copy number. Result(s): The quantitative PCR method showed excellent agreement with the Southern blot analysis. Cases had a median TSPY copy number of 35 (range 20-73), whereas controls had a median TSPY copy number of 34 (range 26-76). This difference was not statistically significant. Conclusion(s): We found no association between TSPY copy numbers and severe spermatogenic failure. The observed variation in TSPY copy number therefore appears to have no functional consequences for semen quality. (Fertil Steril (R) 2010;94:1744-7. (C)2010 by American Society for Reproductive Medicine.)

AB - Objective: To determine whether variation in testis-specific protein Y-encoded (TSPY) gene copy number affects semen quality. Design: Nested case-control study. Setting: University hospital. Patient(s): From a consecutive cohort of 1,016 male partners of subfertile couples, unselected for sperm counts, we selected as cases 100 men with the lowest total number of progressively motile sperm (TMC) and as controls, 100 men with the highest total number of progressively motile sperm. Intervention(s): Quantitative real-time polymerase chain reaction (PCR) and Southern blot to determine TSPY copy number. Main Outcome Measure(s): TSPY copy number. Result(s): The quantitative PCR method showed excellent agreement with the Southern blot analysis. Cases had a median TSPY copy number of 35 (range 20-73), whereas controls had a median TSPY copy number of 34 (range 26-76). This difference was not statistically significant. Conclusion(s): We found no association between TSPY copy numbers and severe spermatogenic failure. The observed variation in TSPY copy number therefore appears to have no functional consequences for semen quality. (Fertil Steril (R) 2010;94:1744-7. (C)2010 by American Society for Reproductive Medicine.)

U2 - https://doi.org/10.1016/j.fertnstert.2009.09.051

DO - https://doi.org/10.1016/j.fertnstert.2009.09.051

M3 - Article

C2 - 19917507

SN - 0015-0282

VL - 94

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JO - Fertility and Sterility

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