TY - JOUR
T1 - β-d-glucosyl-hydroxymethyluracil: A novel modified base present in the DNA of the parasitic protozoan T. brucei
AU - Gommers-Ampt, Janet H.
AU - van Leeuwen, Fred
AU - de Beer, Antonius L. J.
AU - Vliegenthart, Johannes F. G.
AU - Dizdaroglu, Miral
AU - Kowalak, Jeffrey A.
AU - Crain, Pamela F.
AU - Borst, Piet
PY - 1993
Y1 - 1993
N2 - We have previously shown that the DNA of the unicellular eukaryote T. brucel contains about 0.1% of a novel modified base, called J. The presence of J correlates with a DNA modification associated with the silencing of telomeric expression sites for the variant surface antigens of trypanosomes. Here we show that J is 5-((β-d-glucopyranosyloxy)-methyl)-uracil (shortened to β-d-glucosyl-hydroxymethyluracil), a base not previously found in DNA. We discuss putative pathways for the introduction of this base modification at specific positions in the DNA and the possible contribution of this modification to repression of surface antigen gene expression. © 1993.
AB - We have previously shown that the DNA of the unicellular eukaryote T. brucel contains about 0.1% of a novel modified base, called J. The presence of J correlates with a DNA modification associated with the silencing of telomeric expression sites for the variant surface antigens of trypanosomes. Here we show that J is 5-((β-d-glucopyranosyloxy)-methyl)-uracil (shortened to β-d-glucosyl-hydroxymethyluracil), a base not previously found in DNA. We discuss putative pathways for the introduction of this base modification at specific positions in the DNA and the possible contribution of this modification to repression of surface antigen gene expression. © 1993.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0027333558&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/8261512
U2 - https://doi.org/10.1016/0092-8674(93)90322-H
DO - https://doi.org/10.1016/0092-8674(93)90322-H
M3 - Article
C2 - 8261512
SN - 0092-8674
VL - 75
SP - 1129
EP - 1136
JO - Cell
JF - Cell
IS - 6
ER -