TY - JOUR
T1 - A novel flow cytometry-based platelet aggregation assay
AU - De Cuyper, Iris M
AU - Meinders, Marjolein
AU - van de Vijver, Edith
AU - de Korte, Dirk
AU - Porcelijn, Leendert
AU - de Haas, Masja
AU - Eble, Johannes A
AU - Seeger, Karl
AU - Rutella, Sergio
AU - Pagliara, Daria
AU - Kuijpers, Taco W
AU - Verhoeven, Arthur J
AU - van den Berg, Timo K
AU - Gutiérrez, Laura
PY - 2013/3/7
Y1 - 2013/3/7
N2 - The main function of platelets is to maintain normal hemostasis. Inefficient platelet production and/or defective platelet function results in bleeding disorders resulting from a wide range of genetic traits and acquired pathologies. Several platelet function tests have been developed for use in the clinic and in experimental animal models. In particular, platelet aggregation is routinely measured in an aggregometer, which requires normal platelet counts and significant blood sample volumes. For this reason, the analysis of thrombocytopenic patients, infants, and animal models is problematic. We have developed a novel flow cytometry test of platelet aggregation, in which 10- to 25-fold lower platelet counts or sample volumes can be used, either of platelet-rich plasma or whole blood from human subjects or mice. This setup can be applied to test in small assay volumes the influence of a variety of stimuli, drugs, and plasma factors, such as antibodies, on platelet aggregation. The presented principle stands as a novel promising tool, which allows analysis of platelet aggregation in thrombocytopenic patients or infants, and facilitates studies in platelets obtained from experimental animal models without the need of special devices but a flow cytometer.
AB - The main function of platelets is to maintain normal hemostasis. Inefficient platelet production and/or defective platelet function results in bleeding disorders resulting from a wide range of genetic traits and acquired pathologies. Several platelet function tests have been developed for use in the clinic and in experimental animal models. In particular, platelet aggregation is routinely measured in an aggregometer, which requires normal platelet counts and significant blood sample volumes. For this reason, the analysis of thrombocytopenic patients, infants, and animal models is problematic. We have developed a novel flow cytometry test of platelet aggregation, in which 10- to 25-fold lower platelet counts or sample volumes can be used, either of platelet-rich plasma or whole blood from human subjects or mice. This setup can be applied to test in small assay volumes the influence of a variety of stimuli, drugs, and plasma factors, such as antibodies, on platelet aggregation. The presented principle stands as a novel promising tool, which allows analysis of platelet aggregation in thrombocytopenic patients or infants, and facilitates studies in platelets obtained from experimental animal models without the need of special devices but a flow cytometer.
KW - Animals
KW - Autoantibodies/analysis
KW - Blood Platelets/metabolism
KW - Case-Control Studies
KW - Flow Cytometry/methods
KW - Humans
KW - Leukocyte-Adhesion Deficiency Syndrome/blood
KW - Mice
KW - Platelet Activation
KW - Platelet Aggregation
KW - Platelet Count
KW - Platelet-Rich Plasma
KW - Thrombasthenia/blood
U2 - https://doi.org/10.1182/blood-2012-06-437723
DO - https://doi.org/10.1182/blood-2012-06-437723
M3 - Article
C2 - 23303822
SN - 0006-4971
VL - 121
SP - e70-80
JO - Blood
JF - Blood
IS - 10
ER -